Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% SDS-PAGE
- Suitable for: SDS-PAGE, Agglutination, WB
Description
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Product name
Recombinant Influenza A Virus Hemagglutinin H5 protein -
Purity
> 80 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Sequence
mekivllfai vslvksdqic igyhannste qvdtimeknv tvthaqdile kkhngklcdl dgvkplilrd csvagwllgn pmcdefinvp ewsyivekan pvndlcypgd fndyeelkhl lsrinhfeki qiipksswss heaslgvssa cpyqgkssff rnvvwlikkn styptikrsy nntnqedllv lwgihhpnda aeqtklyqnp ttyisvgtst lnqrlvpria trskvngqsg rmeffwtilk pndainfesn gnfiapeyay kivkkgdsti mkseleygnc ntkcqtpmga inssmpfhni hpltigecpk yvksnrlvla tglrnspqre rrrkkrglfg aiagfieggw qgmvdgwygy hhsneqgsgy aadkestqka idgvtnkvns iidkmntqfe avgrefnnle rrienlnkkm edgfldvwty naellvlmen ertldfhdsn vknlydkvrl qlrdnakelg ngcfefyhkc dnecmesvrn gtydypqyse earlkreeis gvklesigiy qilsiystva sslalaimva glslwmcsng slqcrici -
Predicted molecular weight
64 kDa -
Amino acids
1 to 567 -
Additional sequence information
Influenza Hemagglutinin Full Length Glycoprotein (rHA GP) from virus strain A/Vietnam/1203/2004 (H5N1). The theoretical molecular weight of the protein is ~64 kDa, without glycosylation.
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Specifications
Our Abpromise guarantee covers the use of ab190125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Agglutination
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 99% Tris buffered saline
0.01% non-ionic detergent
General Info
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Alternative names
- H5HA
- Hemagglutinin 5
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Relevance
Influenza A belongs to the Orthomyxoviridae family, and is a negative sense single-stranded RNA virus which results in respiratory disease. Haemagglutinin is an antigenic glycoprotein which allows viral attachment and entry into the cell. Sixteen subtypes of haemagglutinin (H1-H16) have been described, of which H1, H2 and H3 infect humans. H5 and H7 normally result in avian disease, with some highly pathogenic H5N1 strains causing 100% mortality in poultry. H5N1 has in some cases mutated to infect humans, with 60% mortality. Haemagglutinin is one of the most medically relevant antigens on influenza as it is a target for antiviral drugs and antibodies.
Images
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anti-H5 ferret antiserum at 1/20 dilution +
Recombinant Influenza A Virus Hemagglutinin H5 protein (ab190125) at 0.2 µg
Secondary
an anti-ferret AP conjugate followed by substrate. -
SDS-PAGE analysis of 1.5 μg of ab190125 protein under reducing conditions.
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Hemagglutination with turkey red blood cells: ab190125 titer 1:8192
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab190125 has been referenced in 4 publications.
- Castellanos-Huerta I et al. Transformation of Dunaliella salina by Agrobacterium tumefaciens for the Expression of the Hemagglutinin of Avian Influenza Virus H5. Microorganisms 10:N/A (2022). PubMed: 35208815
- Bizyaeva AA et al. The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus. Int J Mol Sci 22:N/A (2021). PubMed: 33673708
- Nakakita SI et al. Preparation and Detection of Glycan-Binding Activity of Influenza Virus. Methods Mol Biol 2132:567-583 (2020). PubMed: 32306357
- Novoseltseva AA et al. Structural and Functional Aspects of G-Quadruplex Aptamers Which Bind a Broad Range of Influenza A Viruses. Biomolecules 10:N/A (2020). PubMed: 31936820