Recombinant Anti-NTH1 antibody [EPR15930] (ab191413)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15930] to NTH1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-NTH1 antibody [EPR15930]
See all NTH1 primary antibodies -
Description
Rabbit monoclonal [EPR15930] to NTH1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal heart tissue lysate; HeLa, 293 and K562 cell lysates; Human colon, transitional cell carcinoma of bladder and mouse brain tissues; HeLa cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15930 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191413 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/150.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/150. |
Target
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Function
Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site. Required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. -
Tissue specificity
Widely expressed with highest levels in heart and lowest levels in lung and liver. -
Sequence similarities
Belongs to the Nth/MutY family. -
Developmental stage
Expression levels are regulated during the cell cycle with increased levels during early and mid S-phase. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4913 Human
- Entrez Gene: 18207 Mouse
- Entrez Gene: 29541 Rat
- Omim: 602656 Human
- SwissProt: P78549 Human
- SwissProt: O35980 Mouse
- Unigene: 66196 Human
- Unigene: 148315 Mouse
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Alternative names
- Bifunctional DNA N glycoslyase/DNA (apurinic or apyrimidinic site) lyase antibody
- DNA glycoslyase/AP lyase antibody
- Endonuclease III like protein 1 antibody
see all
Images
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All lanes : Anti-NTH1 antibody [EPR15930] (ab191413) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Human fetal heart lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 34 kDa
Observed band size: 34 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Negative control utilised PBS instead of ab191413.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma of bladder tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Negative control utilised PBS instead of ab191413.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Mouse brain tissue labeling NTH1 with ab191413 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Negative control utilised PBS instead of ab191413.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa cells labeling NTH1 with ab191413 at 1/150 dilution, followed by Goat anti rabbit IgG (Alexa Fluor488) secondary antibody at 1/200 dilution (ab150077). Nuclear counter stain is DAPI (blue).
The two negative controls are ab191413 at 1/250 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) secondary antibody at 1/400 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab191413 has been referenced in 3 publications.
- Tarpey MD et al. Induced in vivo knockdown of the Brca1 gene in skeletal muscle results in skeletal muscle weakness. J Physiol 597:869-887 (2019). PubMed: 30556208
- Li M et al. APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Res 46:5664-5677 (2018). PubMed: 29750271
- Maher RL et al. Human cells contain a factor that facilitates the DNA glycosylase-mediated excision of oxidized bases from occluded sites in nucleosomes. DNA Repair (Amst) 57:91-97 (2017). PubMed: 28709015