Recombinant Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18869] to GABARAPL1 + GABARAPL3
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869]
See all GABARAPL1+GABARAPL3 primary antibodies -
Description
Rabbit monoclonal [EPR18869] to GABARAPL1 + GABARAPL3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human GABARAPL1 full length protein; Recombinant Human GABARAPL3 full length protein; Human fetal brain and fetal liver lysates; HeLa, HepG2, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat brain and kidney lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR18869 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa).
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IP |
1/40.
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Notes |
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WB
1/1000. Detects a band of approximately 17 kDa (predicted molecular weight: 14 kDa). |
IP
1/40. |
Target
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Relevance
GABARAPL1. Function: Ubiquitin-like modifier that increases cell-surface expression of kappa-type opioid receptor through facilitating anterograde intracellular trafficking of the receptor. Involved in formation of autophagosomal vacuoles. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. GABARAPL3. Function: Ubiquitin-like modifier involved in autophagosome formation. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation -
Database links
- Entrez Gene: 23710 Human
- Entrez Gene: 23766 Human
- Entrez Gene: 57436 Mouse
- Entrez Gene: 689161 Rat
- Omim: 607420 Human
- SwissProt: Q9BY60 Human
- SwissProt: Q9H0R8 Human
- SwissProt: Q8R3R8 Mouse
see all -
Alternative names
- Early estrogen-regulated protein antibody
- GABA(A) receptor-associated protein-like 1 antibody
- GABA(A) receptor-associated protein-like 3 antibody
see all
Images
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Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution + Recombinant human GABARAPL1 full length protein at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 43 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
In house recombinant Human GABARAPL1 is a GST-tagged full length protein (aa1-117).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/5000 dilution
Lane 1 : Recombinant Human GABARAPL2 full length protein
Lane 2 : Recombinant Human GABARAPL3 full length protein
Lane 3 : Recombinant Human GABARAP (O95166) full length protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 1 secondBlocking/Dilution buffer: 5% NFDM/TBST.
All three recombinant Human full length proteins contain aa1-117 with an N-terminal His-Tag® and were made in-house.
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 15 seconds; Lane 2,3 and 4: 30 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse kidney tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 5 seconds; Lane 2: 15 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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All lanes : Anti-GABARAPL1 + GABARAPL3 antibody [EPR18869] (ab191887) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat kidney lysate
Lane 3 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 4 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 5 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 14 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 16650615).
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GABARAPL1 + GABARAPL3 was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab191887 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab191887 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10ug (Input).
Lane 2: ab191887 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab191887 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab191887 has not yet been referenced specifically in any publications.