Recombinant Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7787] to TRIM23/ARD1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-TRIM23/ARD1 antibody [EPR7787]
See all TRIM23/ARD1 primary antibodies -
Description
Rabbit monoclonal [EPR7787] to TRIM23/ARD1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, 293T, C6, RAW 264.7, PC.-12 and NIH 3T3 lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7787 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/10000 - 1/50000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
|
Notes |
---|
WB
1/10000 - 1/50000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
Target
-
Function
Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
In the C-terminal section; belongs to the small GTPase superfamily. Arf family.
Contains 1 B box-type zinc finger.
Contains 1 RING-type zinc finger. -
Domain
The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity. -
Cellular localization
Endomembrane system. Golgi apparatus membrane. Lysosome membrane. Membrane-associated with the Golgi complex and lysosomal structures. - Information by UniProt
-
Database links
- Entrez Gene: 373 Human
- Entrez Gene: 81003 Mouse
- Entrez Gene: 81002 Rat
- Omim: 601747 Human
- SwissProt: P36406 Human
- SwissProt: Q8BGX0 Mouse
- SwissProt: P36407 Rat
- Unigene: 792 Human
see all -
Alternative names
- ADP ribosylation factor domain protein 1 antibody
- ADP-ribosylation factor domain-containing protein 1 antibody
- ARF domain protein 1 antibody
see all
Images
-
All lanes : Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032) at 1/50000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 64 kDa -
All lanes : Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032) at 1/10000 dilution
Lane 1 : C6 lysate
Lane 2 : RAW 264.7 lysate
Lane 3 : PC12 lysate
Lane 4 : NIH 3T3 lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 64 kDa
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (1)
ab192032 has been referenced in 1 publication.
- Liu X et al. Disassembly of the TRIM23-TBK1 Complex by the Us11 Protein of Herpes Simplex Virus 1 Impairs Autophagy. J Virol 93:N/A (2019). PubMed: 31189704