Protein G Sepharose® Column (ab193260)
Key features and details
- Sample type: Ascites Fluid, Cell culture media, Cell culture supernatant, Serum
Overview
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Product name
Protein G Sepharose® Column -
Sample type
Cell culture supernatant, Serum, Cell culture media, Ascites Fluid -
Product overview
Protein G Sepharose® Columns are prepared by covalently coupling recombinant Protein G to 6% cross-linked Sepharose® beads. Protein G is a genetically engineered protein containing three IgG-binding regions of native Protein G. The cell wall binding region, albumin binding region and other non-specific regions have been eliminated from the recombinant Protein G to ensure maximum specific IgG binding. The coupling technique is optimized to give a higher binding capacity for IgG and minimum leaching of recombinant Protein G. The IgG binding capacity of Protein G Sepharose® Column is >20 mg of human or rabbit IgG per mL of wet beads.
Protein G Sepharose® Columns display high chemical and physical stability as well as high flow rate, hydrophilicity and high gel strength. This product can be used for IgG purification and immunoprecipitation.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called 6518 Protein G Sepharose Column. 6518-1 is the same size as the 1 ml size of ab193260.
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Tested applications
Suitable for: IP, Purificationmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml 5 ml Protein G Sepharose® Column 1 x 1ml 1 x 5ml -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193260 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
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Purification |
Use at an assay dependent concentration.
Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
Notes |
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IP
Use at an assay dependent concentration. Isolation of antibody/antigen complexes in immunoprecipitation experiments, since only the Fc region is involved in antibody binding and the Fab region is available for binding antigen. |
Purification
Use at an assay dependent concentration. Purification of monoclonal and polyclonal antibodies from culture media, serum, ascites fluid or hybridoma supernatants. |
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab193260 has been referenced in 1 publication.
- Maduwage KP et al. Enzyme immunoassays for detection and quantification of venoms of Sri Lankan snakes: Application in the clinical setting. PLoS Negl Trop Dis 14:e0008668 (2020). PubMed: 33017411