Recombinant Human Metnase protein (ab196433)
Key features and details
- Expression system: Mammalian
- Purity: >= 30% SDS-PAGE
- Tags: DDDDK tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant Human Metnase protein
See all Metnase proteins and peptides -
Purity
>= 30 % SDS-PAGE. -
Expression system
Mammalian -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
QLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQITFPGCI CVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSD HCRNRVVQKGLQFHFQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFS EVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFVDPTYIGNIGRFLNHSC EPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKE RLDHGKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGS APSVFPSCKRLTLETMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAF GPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPL TTTREVAEELNVNHSTVVRHLKQIGKVKKLDKWVPHELTENQKNRRFEVS SSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPIL HPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEIDEMNQKLQRLQLA LVNRKGPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHV FKHLNNFLQGKRFHNQQDAENAFQEFVESQSTDFYATGINQLISRWQKCV DCNGSYFD -
Predicted molecular weight
76 kDa including tags -
Amino acids
27 to 684 -
Tags
DDDDK tag N-Terminus -
Additional sequence information
GenBank accession no.: NM_006515
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab196433 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
The protein fragment was expressed in FreeStyle 293-F cells.
Previously labelled as SETMAR.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C. Avoid freeze / thaw cycle.
pH: 8.00
Constituents: 0.63% Tris HCl, 0.72% Sodium chloride, 0.02% Potassium chloride, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 20% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- Histone lysine N methyltransferase
- Histone lysine N methyltransferase SETMAR
- Hsmar 1
see all -
Function
Histone methyltransferase that methylates 'Lys-4' and 'Lys-36' of histone H3, 2 specific tags for epigenetic transcriptional activation. Specifically mediates dimethylation of H3 'Lys-36'. Has sequence-specific DNA-binding activity and recognizes the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element. Has DNA nicking activity. Has in vivo end joining activity and may mediate genomic integration of foreign DNA. -
Tissue specificity
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle. -
Sequence similarities
In the N-terminal section; belongs to the histone-lysine methyltransferase family.
In the C-terminal section; belongs to the mariner transposase family.
Contains 1 post-SET domain.
Contains 1 pre-SET domain.
Contains 1 SET domain. -
Domain
The mariner transposase Hsmar1 region mediates DNA-binding. It has no transposase activity because the active site contains an Asn in position 610 instead of a Asp residue. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab196433 has not yet been referenced specifically in any publications.