Anti-Insulin Receptor alpha antibody (ab203037)
Key features and details
- Rabbit polyclonal to Insulin Receptor alpha
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Insulin Receptor alpha antibody
See all Insulin Receptor alpha primary antibodies -
Description
Rabbit polyclonal to Insulin Receptor alpha -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human Insulin Receptor alpha aa 700-800 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P06213 -
Positive control
- IHC-P: Rat tongue, brain, lung and small intestine tissue; WB: Human MCF-7, Jurkat, SW480 ad LOVO cell lysates; Flow Cyt (Intra): HL-60 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/100.
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WB |
1/300 - 1/1000. Predicted molecular weight: 156 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/100. |
WB
1/300 - 1/1000. Predicted molecular weight: 156 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
The human insulin receptor is a heterotetrameric membrane glycoprotein consisting of disulfide linked subunits in a beta-alpha-alpha-beta configuration. The beta subunit (95 kDa) possesses a single transmembrane domain, whereas the alpha subunit (135 kDa) is completely extracellular. The insulin receptor exhibits receptor tyrosine kinase (RTK) activity. RTKs are single pass transmembrane receptors that possess intrinsic cytoplasmic enzymatic activity, catalyzing the transfer of the gamma phosphate of ATP to tyrosine residues in protein substrates. RTKs are essential components of signal transduction pathways that affect cell proliferation, differentiation, migration and metabolism. Included in this large protein family are the insulin receptor and the receptors for growth factors such as epidermal growth factor, fibroblast growth factor and vascular endothelial growth factor. Receptor activation occurs through ligand binding, which facilitates receptor dimerization and autophosphorylation of specific tyrosine residues in the cytoplasmic portion. The interaction of insulin with the alpha subunit of the insulin receptor activates the protein tyrosine kinase of the beta subunit, which then undergoes an autophosphorylation that increases its tyrosine kinase activity. Three adapter proteins, IRS1, IRS2 and Shc, become phosphorylated on tyrosine residues following insulin receptor activation. These three phosphorylated proteins then interact with SH2 domain containing signaling proteins. -
Cellular localization
Membrane; single pass type I membrane protein. -
Database links
- Entrez Gene: 3643 Human
- Entrez Gene: 16337 Mouse
- Entrez Gene: 24954 Rat
- Omim: 147670 Human
- SwissProt: P06213 Human
- SwissProt: P15208 Mouse
- SwissProt: P15127 Rat
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Alternative names
- CD220 antibody
- HHF5 antibody
- HIR A antibody
see all
Images
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All lanes : Anti-Insulin Receptor alpha antibody (ab203037) at 1/500 dilution
Lane 1 : Human MCF-7 cell lysate
Lane 2 : Human Jurkat cell lysate
Lane 3 : Human SW480 cell lysate
Lane 4 : Human LOVO cell lysate
Secondary
All lanes : Conjugated secondary antibody for 60 minutes at 37C at 1/20000 dilution
Predicted band size: 156 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat tongue tissue labeling Insulin Receptor with ab203037 at 1/200 dilution, followed by secondary antibody detection and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat small intestine tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
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Intracellular flow cytometric analysis of HL-60 cells labelleing insulin receptor alpha with ab203037 at 1/100 dilution (green), compared to control cells (blue) and isotype control (orange).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat small brain tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling Insulin Receptor alpha with ab203037 at 1/200 dilution, followed by conjugated Goat Anti-Rabbit IgG secondary antibody. DAPI was used as nuclear counterstain. Heat mediated antigen retrieval with sodium citrate buffer, pH 6 was performed for 15 minutes.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab203037 has been referenced in 2 publications.
- Lin L et al. Upregulation of Klotho Aggravates Insulin Resistance in Gestational Diabetes Mellitus Trophoblast Cells. Genet Res (Camb) 2022:1500768 (2022). PubMed: 36325267
- Liu Y et al. D-Mannose Inhibits Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells via the miR669b/MAPK Pathway. Stem Cells Int 2020:8866048 (2020). PubMed: 33376493