Recombinant Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19816] to MLKL (phospho Y222)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MLKL (phospho Y222) antibody [EPR19816]
See all MLKL primary antibodies -
Description
Rabbit monoclonal [EPR19816] to MLKL (phospho Y222) -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19816 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206336 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Sequence similarities
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain. -
Domain
The protein kinase domain is predicted to be catalytically inactive. - Information by UniProt
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Database links
- Entrez Gene: 197259 Human
- Omim: 615153 Human
- SwissProt: Q8NB16 Human
- Unigene: 119878 Human
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Alternative names
- 9130019I15Rik antibody
- FLJ34389 antibody
- hMLKL antibody
see all
Images
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All lanes : Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336) at 1/1000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with MLKL-WT expression vector, whole cell lysate at 20 µg
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate at 4 µg
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate at 20 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate at 20 µg
Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y222F mutant expression vector, whole cell lysate at 20 µg
Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218/222F mutant expression vector, whole cell lysate at 8 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDa
Exposure time: 1 minuteBlocking/Dilution buffer: 5% NFDM/TBST.
Lysates were kindly provided by our collaborator Dr. Xinhua Feng, Zhejiang University.
The molecular weight of NPM-ALK is around 80 kDa (lane 2) [PMID: 11090048].
This antibody can potentially cross react with other kinases.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab206336 has been referenced in 1 publication.
- Mei P et al. E3 ligase TRIM25 ubiquitinates RIP3 to inhibit TNF induced cell necrosis. Cell Death Differ 28:2888-2899 (2021). PubMed: 33953350