Recombinant Anti-IL-16 antibody [EPR19988] (ab207181)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19988] to IL-16
- Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-IL-16 antibody [EPR19988]
See all IL-16 primary antibodies -
Description
Rabbit monoclonal [EPR19988] to IL-16 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IF, IP, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: H9 and Jurkat whole cell lysates; Human thymus, spleen and tonsil lysates. IHC-P: Human tonsil and colon tissues. ICC/IF: H9 cells. Flow Cyt (intra): H9 cells. IP: H9 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19988 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab207181 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 67 kDa.
Observed Mass of target (kDa): 80, 75-40 |
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
1/100.
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IP |
1/30.
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Flow Cyt (Intra) |
1/600.
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Notes |
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WB
1/1000. Predicted molecular weight: 67 kDa. Observed Mass of target (kDa): 80, 75-40 |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
1/100. |
IP
1/30. |
Flow Cyt (Intra)
1/600. |
Target
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Function
Interleukin-16 stimulates a migratory response in CD4+ lymphocytes, monocytes, and eosinophils. Primes CD4+ T-cells for IL-2 and IL-15 responsiveness. Also induces T-lymphocyte expression of interleukin 2 receptor. Ligand for CD4.
Isoform 1 may act as a scaffolding protein that anchors ion channels in the membrane.
Isoform 3 is involved in cell cycle progression in T-cells. Appears to be involved in transcriptional regulation of SKP2 and is probably part of a transcriptional repression complex on the core promoter of the SKP2 gene. May act as a scaffold for GABPB1 (the DNA-binding subunit the GABP transcription factor complex) and HDAC3 thus maintaining transcriptional repression and blocking cell cycle progression in resting T-cells. -
Tissue specificity
Isoform 3 is expressed in hemopoietic tissues, such as resting T-cells, but is undetectable during active T cell proliferation. -
Sequence similarities
Contains 4 PDZ (DHR) domains. -
Post-translational
modificationsIsoform 3 is synthesized as a chemo-attractant inactive precursor in hemopoietic tissues and is proteolytically cleaved by caspase-3 to yield IL-16. -
Cellular localization
Cytoplasm; Cytoplasm. Nucleus and Secreted. - Information by UniProt
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Database links
- Entrez Gene: 3603 Human
- Omim: 603035 Human
- SwissProt: Q14005 Human
- Unigene: 459095 Human
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Alternative names
- HGNC:5980 antibody
- HsT19289 antibody
- IL-16 antibody
see all
Images
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All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution
Lane 1 : H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 67 kDa
Observed band size: 75-40,80 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).
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Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on human tonsil is observed [PMID: 10946273]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16 with ab207181 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on H9 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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All lanes : Anti-IL-16 antibody [EPR19988] (ab207181) at 1/1000 dilution
Lane 1 : Human thymus tissue lysate at 10 µg
Lane 2 : Human spleen tissue lysate at 10 µg
Lane 3 : Human tonsil tissue lysate at 20 µg
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 67 kDa
Observed band size: 75-40,80 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 5 seconds, Lane 2: 15 seconds, Lane 3: 3 minutes.
The band at 80 kDa is pro-IL-16 and the bands at 40-75 kDa are cleaved fragments. This is consistent with what has been described in the literature (PMID: 15187155, PMID: 9144227, PMID: 9743378, PMID: 14734747).
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Immunohistochemical analysis of paraffin-embedded human colon tissue labeling IL-16 with ab207181 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on stromal cells of human colon is observed [PMID: 11709514]. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed H9 (Human cutaneous T lymphocyte lymphoma cell line) cells labeling IL-16with ab207181 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluorr® 488) at 1/2000 dilution was used as the secondary antibody.
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IL-16 was immunoprecipitated from 0.35 mg of H9 (Human cutaneous T lymphocyte lymphoma cell line) whole cell lysate with ab207181 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab207181 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: H9 whole cell lysate, 10 μg (Input).
Lane 2: ab207181 IP in H9 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207181 in H9 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Note: The band at around 50kDa is a cleaved form of IL-16.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab207181 has been referenced in 4 publications.
- Kong X et al. A comprehensive profile of chemokines in the peripheral blood and vascular tissue of patients with Takayasu arteritis. Arthritis Res Ther 24:49 (2022). PubMed: 35172901
- Yang X et al. Rs41291957 polymorphism in the promoter region of microRNA-143 serves as a prognostic biomarker for patients with intracranial hemorrhage. Mol Med Rep 23:N/A (2021). PubMed: 33649782
- Huang Y et al. IL-16 regulates macrophage polarization as a target gene of mir-145-3p. Mol Immunol 107:1-9 (2019). PubMed: 30634164
- Yang WH et al. Heterogeneous nuclear ribonucleoprotein M promotes the progression of breast cancer by regulating the axin/ß-catenin signaling pathway. Biomed Pharmacother 105:848-855 (2018). PubMed: 30021377