Recombinant Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92)
- Suitable for: Flow Cyt (Intra), WB, Dot blot, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-hnRNP A1 (citrulline R92) antibody [EPR20174]
See all hnRNP A1 primary antibodies -
Description
Rabbit monoclonal [EPR20174] to hnRNP A1 (citrulline R92) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, Dot blot, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T transfected with GFP-tagged PADI2 and PADI4, respectively, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysates. Flow Cyt (intra): HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, cells. IP: HEK-293T transfected with GFP-tagged PADI4, treated with 10 mM calcium chloride and 10 µM Ionomycin, whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20174 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab208026 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/600.
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WB |
1/2000. Predicted molecular weight: 39 kDa.
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Dot blot |
1/1000.
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IP |
1/30.
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Notes |
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Flow Cyt (Intra)
1/600. |
WB
1/2000. Predicted molecular weight: 39 kDa. |
Dot blot
1/1000. |
IP
1/30. |
Target
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Function
Involved in the packaging of pre-mRNA into hnRNP particles, transport of poly(A) mRNA from the nucleus to the cytoplasm and may modulate splice site selection. May play a role in HCV RNA replication. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-194, Arg-206 and Arg-225 are dimethylated, probably to asymmetric dimethylarginine.
Sumoylated. -
Cellular localization
Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles continuously between the nucleus and the cytoplasm along with mRNA. Component of ribonucleosomes. In the course of viral infection, colocalizes with HCV NS5B at speckles in the cytoplasm in a HCV-replication dependent manner. - Information by UniProt
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Database links
- Entrez Gene: 3178 Human
- Omim: 164017 Human
- SwissProt: P09651 Human
- Unigene: 546261 Human
- Unigene: 655424 Human
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Alternative names
- HNRNPA 1 antibody
- Helix destabilizing protein antibody
- Helix-destabilizing protein antibody
see all
Images
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All lanes : Anti-hnRNP A1 (citrulline R92) antibody [EPR20174] (ab208026) at 1/2000 dilution
Lanes 1 & 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with a control vector containing GFP tag, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI2 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lane 4 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
According to Uniprot annotation for HNRNPA1, isoform A1-A (34 kDa) is twenty times more abundant than isoform A1-B (39 kDa). The MW of HNRNPA1L2 is also 34 kD.
In WB this antibody does not cross react with CCDC51 (expected MW 45 kDa).
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Dot blot analysis of hnRNP A1 (citrulline R92) labeled with ab208026 at 1/1000 dilution.
Lane 1: hnRNP A1 (citrulline R92) peptide.
Lane 2: hnRNP A1 non-citrulline peptide.
Lane 3: hnRNP A3 (citrulline R113) peptide.
Lane 4: hnRNP A0 (citrulline R85) peptide.
Lane 5: CCDC51(citrulline R142) peptide.
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution was used as secondary antibody.
Based on sequence homology this antibody cross reacts with CCDC51 (citrulline R142) peptide (detected by dot blot only) and hnRNP A1L2 (citrulline R92) protein.
Exposure time: 3 minutes.
Blocking and dilution buffer: 5% NFDM/TBST.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10µM Ionomycin for 2 hours,labeling hnRNP A1 (citrulline R92) with ab208026 at 1/600 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (150079) at 1/2000 dilution was used as the secondary antibody.
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hnRNP A1 (citrulline R92) was immunoprecipitated from 0.35 mg of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate with ab208026 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab208026 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate 10 µg (Input).
Lane 2: ab208026 IP in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab208026 in HEK-293T transfected with GFP-tagged PADI4 (WT) expression vector, treated with 10 mM calcium chloride and 10 µM Ionomycin for 2 hours, whole cell lysate.
Exposure time: Less than 1 second.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab208026 has not yet been referenced specifically in any publications.