Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957)
Key features and details
- Mouse monoclonal [CL3118] to Laminin alpha 5/LAMA5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-Laminin alpha 5/LAMA5 antibody [CL3118]
See all Laminin alpha 5/LAMA5 primary antibodies -
Description
Mouse monoclonal [CL3118] to Laminin alpha 5/LAMA5 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Laminin alpha 5/LAMA5 aa 2550-2650. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O15230 -
Epitope
ab210957 binds to an epitope located within the peptide sequence RQGLVDRAQQ (aa 2555 - 2564). -
Positive control
- Recombinant protein fragment of human Laminin alpha 5/LAMA5; human placenta tissue lysate; human kidney, colon, heart, placenta, stomach, fallopian tube, and cervix tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3118 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab210957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
WB |
1/500 - 1/1000. Predicted molecular weight: 400 kDa.
|
Notes |
---|
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 400 kDa. |
Target
-
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. -
Tissue specificity
Expressed in heart, lung, kidney, skeletal muscle, pancreas, retina and placenta. Little or no expression in brain and liver. -
Sequence similarities
Contains 22 laminin EGF-like domains.
Contains 5 laminin G-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain. -
Domain
Domain G is globular and is part of the major cell-binding site located in the long arm of the laminin heterotrimer. -
Cellular localization
Secreted, extracellular space, extracellular matrix, basement membrane. Major component. - Information by UniProt
-
Database links
- Entrez Gene: 3911 Human
- Omim: 601033 Human
- SwissProt: O15230 Human
- Unigene: 473256 Human
-
Alternative names
- KIAA0533 antibody
- KIAA1907 antibody
- LAMA5 antibody
see all
Images
-
All lanes : Anti-Laminin alpha 5/LAMA5 antibody [CL3118] (ab210957) at 1/500 dilution
Lane 1 : Recombinant protein fragment of Human Laminin 511
Lane 2 : Recombinant protein fragment of Human Laminin 521
Lane 3 : Recombinant protein fragment of Human Laminin 121
Lane 4 : Recombinant protein fragment of Human Laminin 221
Lane 5 : Recombinant protein fragment of Human Laminin 332
Lane 6 : Recombinant protein fragment of Human Laminin 411
Developed using the ECL technique.
Predicted band size: 400 kDa -
Immunohistochemical analysis of paraffin-embedded Human kidney tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human colon tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human heart tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human placenta tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human stomach tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human fallopian tube tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human cervix tissue, labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
-
Immunohistochemical analysis of paraffin-embedded Human lymph nodes tissue (negative control), labeling Laminin alpha 5/LAMA5 using ab210957 at a 1/200 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab210957 has not yet been referenced specifically in any publications.