D 2 Hydroxyglutarate Assay Kit (Colorimetric) (ab211070)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Cell Lysate, Plasma, Serum, Tissue Lysate, Urine
- Sensitivity: 10 µM
Overview
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Product name
D 2 Hydroxyglutarate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Urine, Serum, Plasma, Cell Lysate, Tissue Lysate -
Assay type
Quantitative -
Sensitivity
10 µM -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
D-2-Hydroxyglutarate Assay Kit (ab211070) provides a convenient method to detect D-2-Hydroxyglutarate (D2HG) in urine, cell and tissue lysates. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at OD 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable. It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples. This product is specific for D-2-Hydroxyglutarate and does not detect L-2-Hydroxyglutarate.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K213 D-2-Hydroxyglutarate (D2HG) Assay Kit (Colorimetric). K213-100 is the same size as the 100 test size of ab211070.
In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of αketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG, D-2-Hydroxyglutarate) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Assay Buffer XXXII 1 x 20ml D2HG Enzyme 1 vial D2HG Standard 1 vial Developer Solution III 1 vial
Associated products
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Related Products
Images
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab211070 has been referenced in 13 publications.
- Wu B et al. An Enzymatic Biosensor for the Detection of D-2-Hydroxyglutaric Acid in Serum and Urine. Biosensors (Basel) 12:N/A (2022). PubMed: 35200327
- Sesanto R et al. Low pH Facilitates Heterodimerization of Mutant Isocitrate Dehydrogenase IDH1-R132H and Promotes Production of 2-Hydroxyglutarate. Biochemistry 60:1983-1994 (2021). PubMed: 34143606
- Li B et al. Fresh Tissue Multi-omics Profiling Reveals Immune Classification and Suggests Immunotherapy Candidates for Conventional Chondrosarcoma. Clin Cancer Res N/A:N/A (2021). PubMed: 34426437
- Sears TK et al. IDH1 mutant glioma is preferentially sensitive to the HDAC inhibitor panobinostat. J Neurooncol 154:159-170 (2021). PubMed: 34424450
- Malarz K et al. The Landscape of the Anti-Kinase Activity of the IDH1 Inhibitors. Cancers (Basel) 12:N/A (2020). PubMed: 32110969