Recombinant Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19839] to Prosurfactant Protein C
- Suitable for: WB, IHC-Fr, IP, IHC-P
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-Prosurfactant Protein C antibody [EPR19839]
See all Prosurfactant Protein C primary antibodies -
Description
Rabbit monoclonal [EPR19839] to Prosurfactant Protein C -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Fr, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse lung lysate. IHC-P: Mouse lung tissue. IHC-Fr: Mouse lung tissue. IP: Mouse lung lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19839 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211326 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
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IHC-Fr | (1) |
1/500.
Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
IP |
1/40.
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IHC-P | (2) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). |
IHC-Fr
1/500. Antigen retrieval: Heated citrate solution (10mM citrate PH 6.0 + 0.05% Tween-20). |
IP
1/40. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. -
Involvement in disease
Defects in SFTPC are the cause of pulmonary surfactant metabolism dysfunction type 2 (SMDP2) [MIM:610913]; also called pulmonary alveolar proteinosis due to surfactant protein C deficiency. A rare disease associated with progressive respiratory insufficiency and lung disease with a variable clinical course, due to impaired surfactant homeostasis. It is characterized by alveolar filling with floccular material that stains positive using the periodic acid-Schiff method and is derived from surfactant phospholipids and protein components. Excessive lipoproteins accumulation in the alveoli results in severe respiratory distress.
Genetic variations in SFTPC are a cause of susceptibility to respiratory distress syndrome in premature infants (RDS) [MIM:267450]; also known as RDS in prematurity. RDS is a lung disease affecting usually premature newborn infants. It is characterized by deficient gas exchange, diffuse atelectasis, high-permeability lung edema and fibrin-rich alveolar deposits called 'hyaline membranes'. -
Sequence similarities
Contains 1 BRICHOS domain. -
Cellular localization
Secreted > extracellular space > surface film. - Information by UniProt
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Database links
- Entrez Gene: 20389 Mouse
- SwissProt: P21841 Mouse
- Unigene: 24040 Mouse
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Alternative names
- BRICD6 antibody
- BRICHOS domain containing 6 antibody
- PSP C antibody
see all
Images
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Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 23967208].
The nuclear counterstain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasmic staining on alveolar type II cells of mouse lung is observed [PMID: 15186480].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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All lanes : Anti-Prosurfactant Protein C antibody [EPR19839] (ab211326) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse spleen lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 21 kDa
Additional bands at: 21 kDa (possible isoform), 22 kDa (possible isoform)
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: mouse spleen. [PMID: 15186480].
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SP-C was immunoprecipitated from 0.35 mg of mouse lung lysate with ab211326 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab211326 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution.
Lane 1: Mouse lung lysate, 10ug (Input).
Lane 2: ab211326 IP in mouse lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab211326 in mouse lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
The two bands detected correspond to 22 kda and 21 kda isoforms.
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Prosurfactant Protein C with ab211326 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Negative control: no staining on mouse spleen. [PMID: 15186480]. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (27)
ab211326 has been referenced in 27 publications.
- Fang Y et al. Epithelial Wntless regulates postnatal alveologenesis. Development 149:N/A (2022). PubMed: 34931663
- Wang Z et al. SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages. Cell Mol Immunol 19:210-221 (2022). PubMed: 34983944
- Liu H et al. CoQ10 enhances the efficacy of airway basal stem cell transplantation on bleomycin-induced idiopathic pulmonary fibrosis in mice. Respir Res 23:39 (2022). PubMed: 35219329
- Louie SM et al. Progenitor potential of lung epithelial organoid cells in a transplantation model. Cell Rep 39:110662 (2022). PubMed: 35417699
- Li R et al. Generation of an Ihh-mKate2-Dre knock-in mouse line. Genesis 60:e23488 (2022). PubMed: 35765931