Recombinant Anti-CD31 antibody [EPR17260-254] (ab213175)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-254] to CD31
- Suitable for: WB, IP, Sandwich ELISA
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-CD31 antibody [EPR17260-254]
See all CD31 primary antibodies -
Description
Rabbit monoclonal [EPR17260-254] to CD31 -
Host species
Rabbit -
Specificity
Not suitable for FC-Mouse.
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Tested applications
Suitable for: WB, IP, Sandwich ELISAmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse CD31 active protein; mouse and rat platelet and lung lysates; bEnd.3 whole cell lysate. IP: bEnd.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17260-254 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
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SimpleStep ELISA kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 81 kDa).
Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
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IP |
1/40.
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Sandwich ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 81 kDa). Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
IP
1/40. |
Sandwich ELISA
Use at an assay dependent concentration. |
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 18613 Mouse
- Entrez Gene: 29583 Rat
- SwissProt: Q08481 Mouse
- SwissProt: Q3SWT0 Rat
- Unigene: 343951 Mouse
- Unigene: 1878 Rat
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Anti-CD31 ab213175 was used with Tissue clearing kit – CUBIC (ab316246) and 3D Tissue Staining Kit – CUBIC (ab316248) to penetrate, stain and clear a whole mouse brain.
Learn more about tissue clearing kits, reagents, and protocols designed to make it easier to stain whole brains and get more data from each valuable tissue sample.
For a whole mouse brain, we recommend starting with 10 ug of ab213175 and using a Fab fragment secondary antibody with 6.67 µg to create an antibody complex before 3D staining (see protocol for details). No additive was used during the staining process.
The sample was imaged using a light-sheet microscopy.
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Example of mouse CD31 standard curve in 1X Cell Extraction Buffer PTR using recombinant CD31 antibody (ab213175) and a recombinant monoclonal antibody. Background-subtracted data values (mean +/- SD) are graphed.
For a matched antibody pair set, see Mouse CD31 Matched Antibody Pair Kit (ab212065).
For a complete ELISA kit, see Mouse CD31 ELISA kit (ab204527).
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Lane 1 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/20000 dilution
Lane 2 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/100000 dilution
All lanes : Mouse CD31 active protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 81 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Lanes 1-4 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/1000 dilution
Lane 5 : Anti-CD31 antibody [EPR17260-254] (ab213175) at 1/2000 dilution
Lane 1 : Mouse platelet lysate
Lane 2 : Rat platelet lysate
Lane 3 : Rat lung lysate
Lane 4 : Mouse lung lysate
Lane 5 : bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 81 kDa
Observed band size: 110-130 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1-3: 10 seconds; Lane 4/5: 1 second.
The expression MW observed is consistent with what has been described in the literature (PMID: 18388311; 12433657).
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CD31 was immunoprecipitated from 1 mg of bEnd.3 (Mouse brain endothelioma cell line) whole cell lysate with ab213175 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab213175 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: bEnd.3 whole cell lysate 10µg (Input). Lane 2: ab213175 IP in bEnd.3 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213175 in bEnd.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab213175 has not yet been referenced specifically in any publications.