Recombinant Anti-NOD1 antibody [EPR20833] (ab215726)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20833] to NOD1
- Suitable for: WB
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-NOD1 antibody [EPR20833]
See all NOD1 primary antibodies -
Description
Rabbit monoclonal [EPR20833] to NOD1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild-type BMDM whole cell lysate; mouse lung tissue lysate; mouse spleen tissue lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20833 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa).
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Notes |
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WB
1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 107 kDa). |
Target
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Function
Enhances caspase-9-mediated apoptosis. Induces NF-kappa-B activity via RIPK2 and IKK-gamma. Confers responsiveness to intracellular bacterial lipopolysaccharides (LPS). Forms an intracellular sensing system along with ARHGEF2 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIPK2 dependent NF-kappa-B signaling pathway activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides but also in the activation of NF-kappa-B by Shigella effector proteins IpgB2 and OspB. Recruits NLRP10 to the cell membrane following bacterial infection. -
Tissue specificity
Highly expressed in adult heart, skeletal muscle, pancreas, spleen and ovary. Also detected in placenta, lung, liver, kidney, thymus, testis, small intestine and colon. -
Sequence similarities
Contains 1 CARD domain.
Contains 9 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
Post-translational
modificationsUbiquitinated. 'Lys-48'-linked polyubiquitination by RNF34 promotes proteasomal degradation and thereby negatively regulates NOD1 for instance in NF-kappa-B activition. -
Cellular localization
Cytoplasm. Cell membrane. Apical cell membrane. Basolateral cell membrane. Detected in the cytoplasm and at the cell membrane. Following bacterial infection, localizes to bacterial entry sites in the cell membrane. Recruited to the basolateral and apical membranes in polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 107607 Mouse
- SwissProt: Q8BHB0 Mouse
- Unigene: 28498 Mouse
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Alternative names
- CARD 4 antibody
- CARD4 antibody
- Caspase recruitment domain 4 antibody
see all
Images
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All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution
Lane 1 : Mouse lung lysate boiled before loading
Lane 2 : Mouse lung lysate not boiled before loading
Lane 3 : Mouse spleen lysate boiled before loading
Lane 4 : Mouse spleen lysate not boiled before loading
Lane 5 : Mouse heart lysate boiled before loading
Lane 6 : Mouse heart lysate not boiled before loading
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThe 100-kDa band represents NOD1 monomer and the band above 250 kDa is the aggregates. Other bands are unspecific.
This antibody detects a strong BOI (band of interest) in mouse lung and mouse spleen, but a weak BOI in mouse heart which also expresses NOD1 (PMID:25786158, PMID:28878001, PMID:23028889).
Loading control: Rabbit monoclonal [EPR16891] to GAPDH (ab181602)
Blocking buffer: 5% NFDM/TBST
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Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution + Mouse lung tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 8 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
NOD1 is an alternative name for CARD4.
The 100-kDa band is the specific NOD1 band, other bands are unspecific.
The lysates were un-boiled to avoid the protein aggregation.
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All lanes : Anti-NOD1 antibody [EPR20833] (ab215726) at 1/1000 dilution
Lane 1 : Wild-type BMDM (mouse bone marrow-derived macrophages), whole cell lysate
Lane 2 : NOD1/CARD4 knockout BMDM, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 107 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
The 100-kDa band is the specific NOD1 band, other bands are unspecific.
NOD1 is an alternative name for CARD4.
The WT and NOD1 KO BMDMs lysates were kindly provided by our collaborator Dr. Gang Liu. Tsinghua University.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab215726 has not yet been referenced specifically in any publications.