Anti-S100 beta antibody [S100B/1012] (ab218515)
Key features and details
- Mouse monoclonal [S100B/1012] to S100 beta
- Suitable for: Protein Array, ICC, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-S100 beta antibody [S100B/1012]
See all S100 beta primary antibodies -
Description
Mouse monoclonal [S100B/1012] to S100 beta -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Recombinant full length protein corresponding to Human S100 beta aa 1 to the C-terminus.
Database link: P04271 -
Positive control
- IHC-P: Human melanoma and schwanoma tissues. ICC: Human A2058 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab218515 is purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
S100B/1012 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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ICC |
Use a concentration of 1 - 2 µg/ml.
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IHC-P |
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
ICC
Use a concentration of 1 - 2 µg/ml. |
IHC-P
Use a concentration of 0.25 - 0.5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 525716 Cow
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 100009495 Rabbit
- Entrez Gene: 25742 Rat
- Omim: 176990 Human
- SwissProt: P02638 Cow
- SwissProt: P04271 Human
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling S100 beta with ab218515 at 0.5 µg/mL.
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Immunocytochemistry analysis of A2058 (human epithelial melanoma cell line) cells labeling S100 beta with ab218515 conjugated to AF488 (green). F-actin filaments were labeled with DyLight® 554 Phalloidin (red). DAPI was used to stain the cell nuclei (blue).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human schwanoma tissue labeling S100 beta with ab218515 at 0.5 µg/mL.
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ab218515 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Datasheets and documents
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Datasheet download
References (1)
ab218515 has been referenced in 1 publication.
- Park J et al. A 3D human triculture system modeling neurodegeneration and neuroinflammation in Alzheimer's disease. Nat Neurosci 21:941-951 (2018). Human . PubMed: 29950669