Anti-Clathrin light chain antibody [CLC/1421] (ab219298)
Key features and details
- Mouse monoclonal [CLC/1421] to Clathrin light chain
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-Clathrin light chain antibody [CLC/1421]
See all Clathrin light chain primary antibodies -
Description
Mouse monoclonal [CLC/1421] to Clathrin light chain -
Host species
Mouse -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human Clathrin light chain (N terminal). The exact sequence is proprietary. Purified recombinant N-terminal fragment of human Clathrin light chain (Uniprot P09496 + P09497).
-
Positive control
- Human pancreas tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
CLC/1421 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab219298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
Notes |
---|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
-
Function
Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. -
Cellular localization
Cytoplasmic vesicle membrane. Membrane > coated pit. Cytoplasmic face of coated pits and vesicles. - Information by UniProt
-
Database links
- Entrez Gene: 1211 Human
- Entrez Gene: 1212 Human
- Omim: 118960 Human
- Omim: 118970 Human
- SwissProt: P09496 Human
- SwissProt: P09497 Human
- Unigene: 522114 Human
-
Alternative names
- Clathrin light chain A antibody
- Clathrin light chain B antibody
- Clathrin light chain LCA antibody
see all
Images
Datasheets and documents
-
Datasheet download
References (0)
ab219298 has not yet been referenced specifically in any publications.