Anti-Chromogranin A antibody [CHGA/777] (ab220190)
Key features and details
- Mouse monoclonal [CHGA/777] to Chromogranin A
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Chromogranin A antibody [CHGA/777]
See all Chromogranin A primary antibodies -
Description
Mouse monoclonal [CHGA/777] to Chromogranin A -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat -
Immunogen
Recombinant full length protein corresponding to Human Chromogranin A.
Database link: P10645 -
Positive control
- Human Panc-1 cell lysate;Human pheochromocytoma, pancreas and adrenal gland tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
ab220190 is purified from Bioreactor Concentrate by Protein A/G column chromatography. -
Clonality
Monoclonal -
Clone number
CHGA/777 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220190 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For 30 minutes at RT. |
|
WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa.
|
Notes |
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For 30 minutes at RT. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 50 kDa. |
Target
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Function
Pancreastatin strongly inhibits glucose induced insulin release from the pancreas. -
Sequence similarities
Belongs to the chromogranin/secretogranin protein family. -
Post-translational
modificationsSulfated on tyrosine residues and/or contains sulfated glycans.
O-glycosylated with core 1 or possibly core 8 glycans. -
Cellular localization
Secreted. Neuroendocrine and endocrine secretory granules. - Information by UniProt
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Database links
- Entrez Gene: 1113 Human
- Omim: 118910 Human
- SwissProt: P10645 Human
- Unigene: 150793 Human
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Form
According to the Swiss-Prot database, this protein has many different forms which correspond to various molecular weights; thus the possible variation from the predicted molecular weight of 51kDa. -
Alternative names
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pheochromocytoma tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
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Anti-Chromogranin A antibody [CHGA/777] (ab220190) at 1 µg/ml + Panc-1 cell lysate
Predicted band size: 50 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human pancreas tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human adrenal gland tissue labeling Chromogranin A with ab220190 at 1 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab220190 has not yet been referenced specifically in any publications.