Anti-MMP3 antibody (ab223666)
Key features and details
- Goat polyclonal to MMP3
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MMP3 antibody
See all MMP3 primary antibodies -
Description
Goat polyclonal to MMP3 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Horse, Dog -
Immunogen
Synthetic peptide corresponding to Human MMP3 aa 450 to the C-terminus (C terminal) (Cysteine residue). NP_002413.1
Database link: P08254 -
Positive control
- WB: Mouse eye lysate; human prostate lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 54 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 403445 Dog
- Entrez Gene: 4314 Human
- Entrez Gene: 17392 Mouse
- Entrez Gene: 100009111 Rabbit
- Omim: 185250 Human
- SwissProt: Q6Y4Q5 Dog
- SwissProt: Q28397 Horse
- SwissProt: P08254 Human
see all -
Alternative names
- CHDS6 antibody
- Matrix metalloproteinase 3 antibody
- Matrix metalloproteinase 3 preproprotein antibody
see all
Images
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Anti-MMP3 antibody (ab223666) at 0.3 µg/ml + mouse eye lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour
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Anti-MMP3 antibody (ab223666) at 0.2 µg/ml + human prostate lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour
Datasheets and documents
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Datasheet download
References (0)
ab223666 has not yet been referenced specifically in any publications.