MTS Reagent, cell proliferation assay reagent (ab223881)
Key features and details
- Tetrazolium inner salt; cell proliferation assay reagent
- CAS Number: 138169-43-4
- Purity: > 98%
- Soluble in DPBS to 2 mg/ml
- Form / State: Solid
- Source: Synthetic
Overview
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Product name
MTS Reagent, cell proliferation assay reagent -
Description
Tetrazolium inner salt; cell proliferation assay reagent -
Alternative names
- Owen's Reagent
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Biological description
Tetrazolium inner salt used for determining cell viability in proliferation assays. Reduced in metabolically active cells to a colored formazan product that is soluble in cell culture media. This conversion is thought to be carried out by NAD(P)H-dependent dehydrogenase enzymes.
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Purity
> 98% -
General notes
This product is manufactured by BioVision, an Abcam company and was previously called 2808 MTS Reagent. 2808-250 is the same size as the 250 mg size of ab223881.
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CAS Number
138169-43-4 -
Chemical structure
Properties
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Chemical name
3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium -
Molecular weight
487.51 -
Molecular formula
C20H17N5O6S2 -
PubChem identifier
2762693 -
Storage instructions
Shipped at 4°C. Store at -20°C. Store In the Dark. Store under desiccating conditions. -
Solubility overview
Soluble in DPBS to 2 mg/ml -
Handling
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES
CC1=C(SC(=N1)[N+]2=NC(=NN2C3=CC=C(C=C3)S(=O)(=O)[O-])C4=CC(=CC=C4)OCC(=O)O)C -
Source
Synthetic
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab223881 has been referenced in 4 publications.
- Hernandez-Espinosa DR et al. Microglial reprogramming by Hv1 antagonism protects neurons from inflammatory and glutamate toxicity. J Neurochem 165:29-54 (2023). PubMed: 36625847
- Chulpanova DS et al. Cytochalasin B-Induced Membrane Vesicles from TRAIL-Overexpressing Mesenchymal Stem Cells Induce Extrinsic Pathway of Apoptosis in Breast Cancer Mouse Model. Curr Issues Mol Biol 45:571-592 (2023). PubMed: 36661524
- Najer A et al. Potent Virustatic Polymer-Lipid Nanomimics Block Viral Entry and Inhibit Malaria Parasites In Vivo. ACS Cent Sci 8:1238-1257 (2022). PubMed: 36188342
- Wang Y et al. Cytohesin-2/ARNO: A Novel Bridge Between Cell Migration and Immunoregulation in Synovial Fibroblasts. Front Immunol 12:809896 (2021). PubMed: 35095899