Anti-cGAS antibody (ab224144)
Key features and details
- Rabbit polyclonal to cGAS
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-cGAS antibody
See all cGAS primary antibodies -
Description
Rabbit polyclonal to cGAS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human cGAS aa 300-450.
Database link: Q8N884 -
Positive control
- WB: CACO-2 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224144 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa. |
Target
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Function
Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2',5' phosphodiester linkage at the GpA step and the formation of a 3',5' phosphodiester linkage at the ApG step, producing c[G(2',5')pA(3',5')p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. -
Tissue specificity
Expressed in the monocytic cell line THP1. -
Sequence similarities
Belongs to the mab-21 family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 115004 Human
- Omim: 613973 Human
- SwissProt: Q8N884 Human
- Unigene: 658405 Human
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Alternative names
- C6orf150 antibody
- cGAMP synthase antibody
- cGAS antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab224144 has been referenced in 4 publications.
- Vicencio JM et al. Osimertinib and anti-HER3 combination therapy engages immune dependent tumor toxicity via STING activation in trans. Cell Death Dis 13:274 (2022). PubMed: 35347108
- Zhou M et al. Activation of cGAS-STING pathway - A possible cause of myofiber atrophy/necrosis in dermatomyositis and immune-mediated necrotizing myopathy. J Clin Lab Anal 36:e24631 (2022). PubMed: 36030554
- Zhang YN et al. Increased cGAS/STING signaling components in patients with Mooren's ulcer. Int J Ophthalmol 14:1660-1665 (2021). PubMed: 34804854
- Cindrova-Davies T et al. Evidence of oxidative stress-induced senescence in mature, post-mature and pathological human placentas. Placenta 68:15-22 (2018). PubMed: 30055665