Anti-TRIOBP antibody (ab224226)
Key features and details
- Rabbit polyclonal to TRIOBP
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TRIOBP antibody
See all TRIOBP primary antibodies -
Description
Rabbit polyclonal to TRIOBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Recombinant fragment corresponding to Human TRIOBP aa 2200-2350.
Database link: Q9H2D6 -
Positive control
- WB: PC-3, U-87 MG and Caco-2 cell lysates. IHC-P: Human heart muscle tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224226 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 261 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 261 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Relevance
TRIOBP is a protein that interacts with trio, which is involved with neural tissue development and controlling actin cytoskeleton organization, cell motility and cell growth. This trio binding protein also associates with F actin and stabilizes F actin structures. -
Cellular localization
Nucleus. Cytoplasm; cytoskeleton. Note: Localized to F-actin in a periodic pattern. -
Database links
- Entrez Gene: 11078 Human
- Entrez Gene: 110253 Mouse
- Omim: 609761 Human
- SwissProt: Q9H2D6 Human
- SwissProt: Q99KW3 Mouse
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Alternative names
- DFNB28 antibody
- KIAA1662 antibody
- Protein TARA antibody
see all
Images
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Paraffin-embedded human heart muscle tissue stained for TRIOBP using ab224226 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-TRIOBP antibody (ab224226) at 1/100 dilution
Lane 1 : PC-3 (human prostate adenocarcinoma cell line) cell lysate
Lane 2 : Caco-2 (human colorectal adenocarcinoma cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 261 kDa
Additional bands at: 74 kDa (possible isoform) -
Anti-TRIOBP antibody (ab224226) at 1/100 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Developed using the ECL technique.
Predicted band size: 261 kDa
Additional bands at: 74 kDa (possible isoform)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab224226 has been referenced in 1 publication.
- Han Y et al. Tumor-Induced Generation of Splenic Erythroblast-like Ter-Cells Promotes Tumor Progression. Cell 173:634-648.e12 (2018). PubMed: 29606356