Recombinant Anti-Factor XIIIa antibody [SP196] (ab225722)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP196] to Factor XIIIa
- Suitable for: IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Factor XIIIa antibody [SP196]
See all Factor XIIIa primary antibodies -
Description
Rabbit monoclonal [SP196] to Factor XIIIa -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human placenta, colon, HK lymphoma, esophagus, bladder, lung, skin, skin squamous cell carcinoma, and " Human Hodgkins lymphoma" tissue.
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General notes
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP196 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab225722 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 20 minutes at room temperature. |
Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol. Primary incubation for 20 minutes at room temperature. |
Target
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Function
Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. -
Involvement in disease
Defects in F13A1 are the cause of factor XIII subunit A deficiency (FA13AD) [MIM:613225]. FA13AD is an autosomal recessive disorder characterized by a life-long bleeding tendency, impaired wound healing and spontaneous abortion in affected women. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsThe activation peptide is released by thrombin. -
Cellular localization
Cytoplasm. Secreted. Secreted into the blood plasma. Cytoplasmic in most tissues, but also secreted in the blood plasma. - Information by UniProt
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Database links
- Entrez Gene: 2162 Human
- Omim: 134570 Human
- SwissProt: P00488 Human
- Unigene: 335513 Human
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Alternative names
- bA525O21.1 (coagulation factor XIII, A1 polypeptide) antibody
- Coagulation factor XIII A chain antibody
- Coagulation factor XIII A1 polypeptide antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human Hodgkin's lymphoma tissue sections labeling Factor XIIIa with ab225722 at 1/100 dilution (1.22µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Factor XIIIa with ab225722 at 1/100 dilution (1.22 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Formalin-fixed, paraffin-embedded human placenta tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human colon tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human esophagus tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human bladder tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human lung tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for Factor XIIIa using ab225722 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab225722 has not yet been referenced specifically in any publications.