Anti-Glucocorticoid Receptor antibody (ab227524)
Key features and details
- Rabbit polyclonal to Glucocorticoid Receptor
- Suitable for: WB, IP, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Glucocorticoid Receptor antibody
See all Glucocorticoid Receptor primary antibodies -
Description
Rabbit polyclonal to Glucocorticoid Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Cow, Cat -
Immunogen
Recombinant fragment within Human Glucocorticoid Receptor (internal sequence). The exact sequence is proprietary.
Database link: P04150 -
Positive control
- WB: HeLa whole cell extract; Jurkat and Raji whole cell lysates. IP: HeLa whole cell extract. IHC-P: Human colon carcinoma tissue. ICC/IF: A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 86 kDa.
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IP |
1/500 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 86 kDa. |
IP
1/500 - 1/1000. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE) and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation. Involved in nuclear translocation. -
Tissue specificity
Widely expressed. In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node as well as whole adult and fetal heart. -
Involvement in disease
Defects in NR3C1 are a cause of glucocorticoid resistance (GCRES) [MIM:138040]; also known as cortisol resistance. It is a hypertensive, hyperandrogenic disorder characterized by increased serum cortisol concentrations. Inheritance is autosomal dominant. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Domain
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
Post-translational
modificationsIncreased proteasome-mediated degradation in response to glucocorticoids.
Phosphorylated in the absence of hormone; becomes hyperphosphorylated in the presence of glucocorticoid. The Ser-203-phosphorylated form is mainly cytoplasmic, and the Ser-211-phosphorylated form is nuclear. Transcriptional activity correlates with the amount of phosphorylation at Ser-211.
Sumoylated; this reduces transcription transactivation.
Ubiquitinated; restricts glucocorticoid-mediated transcriptional signaling. -
Cellular localization
Cytoplasm. Nucleus. Cytoplasmic in the absence of ligand, nuclear after ligand-binding and Nucleus. Localized largely in the nucleus. - Information by UniProt
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Database links
- Entrez Gene: 2908 Human
- Omim: 138040 Human
- SwissProt: P04150 Human
- Unigene: 122926 Human
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Alternative names
- GCCR antibody
- GCR antibody
- GCR_HUMAN antibody
see all
Images
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Glucocorticoid Receptor Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 86 kDa -
All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : Glucocorticoid Receptor knockout HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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All lanes : Anti-Glucocorticoid Receptor antibody (ab227524) at 1/1000 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 86 kDa7.5% SDS-PAGE gel.
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Paraffin-embedded human colon carcinoma tissue stained for Glucocorticoid Receptor using ab227524 at 1/250 dilution in immunohistochemical analysis.
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Paraformaldehyde-fixed A431 (human epidermoid carcinoma cell line) cells stained for Glucocorticoid Receptor (green) using ab227524 at 1/200 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
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Glucocorticoid Receptor was immunoprecipitated from 1 mg HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with 2.5 µg of ab227524. Western blot was performed from the immunoprecipitate using ab227524 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate 40 µg (Input).
Lane 2: 2.5 µg preimmune rabbit IgG instead of ab227524 in HeLa whole cell lysate.
Lane 3: ab227524 IP in HeLa whole cell lysate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227524 has not yet been referenced specifically in any publications.