Anti-RFRP antibody (ab228496)
Key features and details
- Guinea pig polyclonal to RFRP
- Suitable for: IHC-Fr
- Reacts with: Sheep
- Isotype: IgG
Overview
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Product name
Anti-RFRP antibody
See all RFRP primary antibodies -
Description
Guinea pig polyclonal to RFRP -
Host species
Guinea pig -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Sheep
Predicted to work with: Human -
Immunogen
Synthetic peptide corresponding to Human RFRP aa 100 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: Q9HCQ7 -
Positive control
- IHC-P: Sheep hypothalamus tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Lyophilized:Reconstitute in 50 µl sterile water. Centrifuge to remove any insoluble material. After reconstitution, aliquot and store at -20°C for a higher stability. -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7 -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
1/2000.
|
Notes |
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IHC-Fr
1/2000. |
Target
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Function
Neuropeptide RFRP-1 acts as a potent negative regulator of gonadotropin synthesis and secretion. Neuropeptides NPSF and NPVF efficiently inhibit forskolin-induced production of cAMP, but RFRP-2 shows no inhibitory activity. Neuropeptide RFRP-1 induces secretion of prolactin in rats. Neuropeptide NPVF blocks morphine-induced analgesia. -
Tissue specificity
Isoform 1 is specifically expressed in the retina. Neuropeptides RFRP-1 and NPVF are detected in the hypothalamus. -
Sequence similarities
Belongs to the FARP (FMRFamide related peptide) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 64111 Human
- SwissProt: Q9HCQ7 Human
- Unigene: 60473 Human
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Alternative names
- C7orf9 antibody
- Neuropeptide NPSF antibody
- Neuropeptide NPVF antibody
see all
Images
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4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis.
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4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Kisspeptin.
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4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with Prolactin-releasing peptide (PrRP).
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4% PFA-fixed frozen sheep hypothalamus tissue stained for RFRP (green) using ab228496 at 1/2000 dilution in immunohistochemical analysis. Staining is not affected by preadsorption with NFF peptide.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab228496 has not yet been referenced specifically in any publications.