Recombinant Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22904-135] to SLAM / CD150
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-SLAM / CD150 antibody [EPR22904-135]
See all SLAM / CD150 primary antibodies -
Description
Rabbit monoclonal [EPR22904-135] to SLAM / CD150 -
Host species
Rabbit -
Specificity
IHC-P is recommended for mouse only.
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Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse thymus and spleen lysates; rat thymus lysate. IHC-P: Mouse spleen and bone marrow tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22904-135 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab228978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Recommended for mouse only. |
Notes |
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WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 38 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. Recommended for mouse only. |
Target
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Function
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
Tissue specificity
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Domain
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
Post-translational
modificationsPhosphorylated by FYN. -
Cellular localization
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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Database links
- Entrez Gene: 27218 Mouse
- SwissProt: Q9QUM4 Mouse
- Unigene: 103648 Mouse
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Alternative names
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse bone marrow tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
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All lanes : Anti-SLAM / CD150 antibody [EPR22904-135] (ab228978) at 1/1000 dilution
Lane 1 : Mouse thymus lysate
Lane 2 : Mouse spleen lysate
Lane 3 : Mouse lung lysate
Lane 4 : Rat thymus lysate
Lane 5 : Rat lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 38 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure times.
Lane 1: 26 seconds; Lanes 2-5: 136 seconds.
The molecular weight observed is consistent with what has been described in the literature (PMID: 28982149).
Negative control: Lung (PMID: 11950898)
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Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SLAM /CD150 with ab228978 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The section was incubated with ab228978 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Positive staining on megakaryocytes in mouse bone marrow (PMID: 15989959) is observed. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab228978 has been referenced in 1 publication.
- Qi W & Zhang Q Identification and Validation of Immune Molecular Subtypes and Immune Landscape Based on Colon Cancer Cohort. Front Med (Lausanne) 9:827695 (2022). PubMed: 35602471