Recombinant Anti-GALC antibody [EPR23598-126] (ab240638)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23598-126] to GALC
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-GALC antibody [EPR23598-126]
See all GALC primary antibodies -
Description
Rabbit monoclonal [EPR23598-126] to GALC -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: PC-12 whole cell lysate; Mouse brain and testis lysates; Rat brain and testis lysates; Human brain lysate. IHC-P: Human cerebrum tissue; Mouse cerebrum tissue; Rat cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23598-126 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 77 kDa.
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IHC-P |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Predicted molecular weight: 77 kDa. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Hydrolyzes the galactose ester bonds of galactosylceramide, galactosylsphingosine, lactosylceramide, and monogalactosyldiglyceride. Enzyme with very low activity responsible for the lysosomal catabolism of galactosylceramide, a major lipid in myelin, kidney and epithelial cells of small intestine and colon. -
Tissue specificity
Detected in urine. Detected in testis, brain and placenta (at protein level). Detected in kidney and liver. -
Involvement in disease
Leukodystrophy, globoid cell -
Sequence similarities
Belongs to the glycosyl hydrolase 59 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 2581 Human
- Entrez Gene: 14420 Mouse
- Entrez Gene: 314360 Rat
- Omim: 606890 Human
- SwissProt: P54803 Human
- SwissProt: P54818 Mouse
- Unigene: 513439 Human
- Unigene: 5120 Mouse
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Alternative names
- Gacy antibody
- Galactocerebrosidase antibody
- Galactocerebroside beta galactosidase antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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All lanes : Anti-GALC antibody [EPR23598-126] (ab240638) at 1/1000 dilution
Lane 1 : PC-12 (rat adrenal gland pheochromocytoma ) whole cell lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Mouse testis tissue lysate
Lane 5 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution
Predicted band size: 77 kDa
Observed band size: 140,50,70,80 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The precursor form of GALC (80 KD) is processed in lysosomes into N-terminal fragment (70 and 50 KD) and C-terminal fragment (30 KD), the observed band at 140 KD is the aggregate GALC, which is consistent to the literature(PMID: 7852280;PMID: 9760019;PMID: 20410102).
Exposure time: 37 seconds.
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Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
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Anti-GALC antibody [EPR23598-126] (ab240638) at 1/1000 dilution + Human brain tissue lysate at 20 µg
Secondary
VeriBlot for IP secondary antibody(HRP)(ab131366) at 1/1000 dilution
Predicted band size: 77 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST This blot was developed using a higher sensitivity ECL substrate.
The precursor form of GALC (80 KD) is processed in lysosomes into N-terminal fragment (50 KD), which is consistent to the literature(PMID: 7852280;PMID: 9760019).
Exposure time: 3 minutes.
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Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling GALC with ab240638 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human cerebrum (PMID: 15987783). The section was incubated with ab240638 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab240638 has not yet been referenced specifically in any publications.