Anti-LDL Receptor antibody (ab240748)
Key features and details
- Rabbit polyclonal to LDL Receptor
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-LDL Receptor antibody
See all LDL Receptor primary antibodies -
Description
Rabbit polyclonal to LDL Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Cow -
Immunogen
Synthetic peptide within Human LDL Receptor aa 810-860. The exact sequence is proprietary. (NP_000518.1).
Database link: P01130 -
Positive control
- IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab240748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. -
Involvement in disease
Defects in LDLR are the cause of familial hypercholesterolemia (FH) [MIM:143890]; a common autosomal semi-dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). -
Sequence similarities
Belongs to the LDLR family.
Contains 3 EGF-like domains.
Contains 7 LDL-receptor class A domains.
Contains 6 LDL-receptor class B repeats. -
Post-translational
modificationsN- and O-glycosylated.
Ubiquitinated by MYLIP leading to degradation. -
Cellular localization
Cell membrane. Endomembrane system. Membrane > clathrin-coated pit. Found distributed from the plasma membrane to intracellular compartments. - Information by UniProt
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Database links
- Entrez Gene: 3949 Human
- Entrez Gene: 16835 Mouse
- Entrez Gene: 300438 Rat
- Omim: 606945 Human
- SwissProt: P01130 Human
- SwissProt: P35951 Mouse
- SwissProt: P35952 Rat
- Unigene: 213289 Human
see all -
Alternative names
- FH antibody
- FHC antibody
- LDL R antibody
see all
Images
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LDL Receptor was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab240748 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab240748 at 1 µg/ml.
Lane 1: ab240748 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.Detection: Chemiluminescence with exposure time of 3 minutes.
Lysate prepared using NETN buffer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab240748 has not yet been referenced specifically in any publications.