Recombinant Anti-CDKN2A/p16INK4a antibody [PABLO33B] (ab241543)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a
- Suitable for: IHC-P
- Knockout validated
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CDKN2A/p16INK4a antibody [PABLO33B]
See all CDKN2A/p16INK4a primary antibodies -
Description
Rat monoclonal [PABLO33B] to CDKN2A/p16INK4a -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Wild type mouse fibrosarcoma tissue. Mouse papilloma tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59.04% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
PABLO33B -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab241543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/640.
|
Notes |
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IHC-P
1/640. |
Target
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Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 12578 Mouse
- SwissProt: P51480 Mouse
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Form
There are 4 isoforms produced by alternative splicing. Isoform 1 also known as: p16INK4a; Isoform 3 also known as: p12; Isoform 4 also known as: p14ARF; p19ARF; ARF. -
Alternative names
- CCM2 antibody
- CDK4 inhibitor p16 INK4 antibody
- CDK4I antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Wild type mouse fibrosarcoma and p16 knock-out mouse fibrosarcoma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 µg/mL).
Image A: Positive staining on the wild type mouse fibrosarcoma
Image B: No staining on the p16 knock-out mouse fibrosarcoma
The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
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Immunohistochemical analysis of paraffin-embedded mouse papilloma tissue labeling CDKN2A/p16INK4a with ab241543 at 1/640 dilution (1.28 µg/mL). Positive staining on the mouse papilloma. The experiment was done in the Discovery XT (Ventana-Rache) machine using the CC1 standard buffer. The secondary antibody was a Rabbit a-rat Biotinylated followed by OmniMap anti rabbit. Counterstained with Hematoxylin.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab241543 has been referenced in 2 publications.
- Li J et al. TGFβ1+CCR5+ neutrophil subset increases in bone marrow and causes age-related osteoporosis in male mice. Nat Commun 14:159 (2023). PubMed: 36631487
- Ji ML et al. Sirt6 attenuates chondrocyte senescence and osteoarthritis progression. Nat Commun 13:7658 (2022). PubMed: 36496445