Recombinant Anti-ZBTB20 antibody [4A3] (ab243143)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [4A3] to ZBTB20
- Suitable for: IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-ZBTB20 antibody [4A3]
See all ZBTB20 primary antibodies -
Description
Rat monoclonal [4A3] to ZBTB20 -
Host species
Rat -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment (His-tag) corresponding to Mouse ZBTB20.
Database link: Q8K0L9 -
Positive control
- IHC-P: Mouse E14.5 cerebrum tissue. Mouse and rat hippocampus. Mouse pancreas tissue.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Ion Exchange Chromatography -
Clonality
Monoclonal -
Clone number
4A3 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 0.077 mg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 0.077 mg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
May be a transcription factor that may be involved in hematopoiesis, oncogenesis, and immune responses. -
Tissue specificity
Expressed in spleen, lymph node, thymus, peripheral blood leukocytes, and fetal liver. -
Sequence similarities
Contains 1 BTB (POZ) domain.
Contains 5 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 56490 Mouse
- SwissProt: Q8K0L9 Mouse
- Unigene: 136238 Mouse
- Unigene: 440824 Mouse
- Unigene: 441121 Mouse
- Unigene: 441972 Mouse
- Unigene: 460947 Mouse
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Alternative names
- Dendritic derived BTB/POZ zinc finger protein antibody
- Dendritic-derived BTB/POZ zinc finger protein antibody
- DKFZp566F123 antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse E14.5 cerebrum tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse E14.5 cerebrum(PMID: 20308569). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse pancreas (PMID: 22374165). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on mouse hippocampus (PMID: 11744704). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue labeling ZBTB20 with ab243143 at 0.077µg/ml, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on rat hippocampus (PMID: 11744704). Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, followed by ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243143 has been referenced in 1 publication.
- Li F et al. Zinc Finger Protein ZBTB20 protects against cardiac remodelling post-myocardial infarction via ROS-TNFa/ASK1/JNK pathway regulation. J Cell Mol Med 24:13383-13396 (2020). PubMed: 33063955