Recombinant Anti-CD4 antibody [BL-155-1C11] - BSA free (ab243872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BL-155-1C11] to CD4 - BSA free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
-
Product name
Anti-CD4 antibody [BL-155-1C11] - BSA free
See all CD4 primary antibodies -
Description
Rabbit monoclonal [BL-155-1C11] to CD4 - BSA free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD4 (extracellular).
Database link: P01730 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading...
-
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BL-155-1C11 -
Isotype
IgG -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000.
|
|
IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
IP |
Use at an assay dependent concentration.
Use 20µl/mg lysate. |
Notes |
---|
WB
1/1000. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IP
Use at an assay dependent concentration. Use 20µl/mg lysate. |
Target
-
Function
Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. -
Sequence similarities
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsPalmitoylation and association with LCK contribute to the enrichment of CD4 in lipid rafts. -
Cellular localization
Cell membrane. Localizes to lipid rafts. Removed from plasma membrane by HIV-1 Nef protein that increases clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation. Cell surface expression is also down-modulated by HIV-1 Envelope polyprotein gp160 that interacts with, and sequesters CD4 in the endoplasmic reticulum. - Information by UniProt
-
Database links
- Entrez Gene: 920 Human
- Omim: 186940 Human
- SwissProt: P01730 Human
- Unigene: 631659 Human
-
Alternative names
- CD 4 antibody
- CD4 (L3T4) antibody
- CD4 antibody
see all
Images
-
CD4 was immunoprecipitated from 1 mg MOLT-4 whole cell lysate with ab243872 at 20 µl per reaction Western blot was performed on the immunoprecipitate using ab243872 at 1/1000 dilution.
Lane 1: ab243872 IP in MOLT-4 cell lysate.
Lane 2: Contol IgG in MOLT-4 cell lysate.
Detection: Chemiluminescence with an exposure time of 3 minutes
-
Western blot analysis using ab243872 at 1/1000 dilution.
Lane 1: Jurkat whole cell lysate (15 µg).
Lane 2: MOLT-4 whole cell lysate (15 µg).
Lane3: U-937 whole cell lysate (15 µg).
Lane 4: Ramos whole cell lysate (15 µg).
Lane 5: SR whole cell lysate (15 µg).
Lane 6: HL-60 whole cell lysate (15 µg).
Lane 7: HeLa whole cell lysate (15µg).
Lane 8:HEK-293T whole cell lysate (15 µg).
Lane 9: HEPG2 whole cell lysate (15 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 30 seconds
-
Formalin-fixed, paraffin-embedded human appendix tissue stained for CD4 using ab243872 at 1/200 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab243872 has not yet been referenced specifically in any publications.