Anti-CD44 antibody (ab24504)
Key features and details
- Rabbit polyclonal to CD44
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD44 antibody
See all CD44 primary antibodies -
Description
Rabbit polyclonal to CD44 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human CD44.
(Peptide available asab52122) -
Positive control
- WB: Mouse small intestine tissue lysate
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3393 HCAM Antibody. 3393-100 is the same size as the 100 µg size of ab24504.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab24504 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 82 kDa.
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Notes |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 82 kDa. |
Target
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Function
Receptor for hyaluronic acid (HA). Mediates cell-cell and cell-matrix interactions through its affinity for HA, and possibly also through its affinity for other ligands such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Adhesion with HA plays an important role in cell migration, tumor growth and progression. Also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. Altered expression or dysfunction causes numerous pathogenic phenotypes. Great protein heterogeneity due to numerous alternative splicing and post-translational modification events. -
Tissue specificity
Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells. -
Sequence similarities
Contains 1 Link domain. -
Domain
The lectin-like LINK domain is responsible for hyaluronan binding. -
Post-translational
modificationsProteolytically cleaved in the extracellular matrix by specific proteinases (possibly MMPs) in several cell lines and tumors.
N-glycosylated.
O-glycosylated; contains more-or-less-sulfated chondroitin sulfate glycans, whose number may affect the accessibility of specific proteinases to their cleavage site(s).
Phosphorylated; activation of PKC results in the dephosphorylation of Ser-706 (constitutive phosphorylation site), and the phosphorylation of Ser-672. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 12505 Mouse
- SwissProt: P15379 Mouse
- Unigene: 423621 Mouse
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Alternative names
- LHR antibody
- BA-1 antibody
- CD 44 antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab24504 has been referenced in 31 publications.
- Ishii H et al. Isolation and characterization of cancer stem cells derived from human glioblastoma. Am J Cancer Res 11:441-457 (2021). PubMed: 33575080
- Liguori M et al. The soluble glycoprotein NMB (GPNMB) produced by macrophages induces cancer stemness and metastasis via CD44 and IL-33. Cell Mol Immunol 18:711-722 (2021). PubMed: 32728200
- van den Berg NWE et al. Epicardial and endothelial cell activation concurs with extracellular matrix remodeling in atrial fibrillation. Clin Transl Med 11:e558 (2021). PubMed: 34841686
- Sun XY et al. Regulatory Effects of Damaged Renal Epithelial Cells After Repair by Porphyra yezoensis Polysaccharides with Different Sulfation Degree on the Calcium Oxalate Crystal-Cell Interaction. Int J Nanomedicine 16:8087-8102 (2021). PubMed: 34934314
- Guillamat-Prats R et al. Alveolar Type II Cells or Mesenchymal Stem Cells: Comparison of Two Different Cell Therapies for the Treatment of Acute Lung Injury in Rats. Cells 9:N/A (2020). PubMed: 32751857