Recombinant Anti-CIRP antibody [EPR23628-36] (ab246510)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23628-36] to CIRP
- Suitable for: WB, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-CIRP antibody [EPR23628-36]
See all CIRP primary antibodies -
Description
Rabbit monoclonal [EPR23628-36] to CIRP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: NIH/3T3 and NIH/3T3 treated at 32°C for 48 hours, whole cell lysates; Mouse testis tissue lysate; Rat testis tissue lysate. IP: Mouse testis tissue lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23628-36 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (2) |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
|
IP |
1/30.
|
Notes |
---|
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa). |
IP
1/30. |
Target
-
Function
Cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival. Acts as a translational activator. Seems to play an essential role in cold-induced suppression of cell proliferation. Binds specifically to the 3'-untranslated regions (3'-UTRs) of stress-responsive transcripts RPA2 and TXN. Acts as a translational repressor (By similarity). Promotes assembly of stress granules (SGs), when overexpressed. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. -
Domain
Both the RRM domain and the arginine, glycine (RGG) rich domain are necessary for binding to the TXN 3'-untranslated region. Both the RRM domain and the arginine, glycine (RGG) rich domain (RGG repeats) are necessary for optimal recruitment into SGs upon cellular stress. The C-terminal domain containing RGG repeats is necessary for translational repression. -
Post-translational
modificationsMethylated on arginine residues. Methylation of the RGG motifs is a prerequisite for recruitment into SGs.
Phosphorylated by CK2, GSK3A and GSK3B. Phosphorylation by GSK3B increases RNA-binding activity to the TXN 3'-UTR transcript upon exposure to UV radiation. -
Cellular localization
Nucleus > nucleoplasm. Cytoplasm. Translocates from the nucleus to the cytoplasm after exposure to UV radiation. Translocates from the nucleus to the cytoplasm into stress granules upon various cytoplasmic stresses, such as osmotic and heat shocks. Its recruitment into stress granules occurs in the absence of TIAR proteins. - Information by UniProt
-
Database links
- Entrez Gene: 12696 Mouse
- Entrez Gene: 81825 Rat
- SwissProt: P60824 Mouse
- SwissProt: P60825 Rat
- Unigene: 17898 Mouse
- Unigene: 28931 Rat
-
Alternative names
- A18 hnRNP antibody
- A18HNRNP antibody
- cirbp antibody
see all
Images
-
All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) treated with 32°C for 48 h whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa
Exposure time: 180 secondsBlocking and dilution buffer: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate.
-
All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Rat testis tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
-
CIRBP was immunoprecipitated from 0.35 mg Mouse testis tissue lysate with ab246510 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246510 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse testis tissue lysate 10ug
Lane 2: ab246510 IP in Mouse testis tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246510 in Mouse testis tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 47 seconds.
This blot was developed using a higher sensitivity ECL substrate.
-
All lanes : Anti-CIRP antibody [EPR23628-36] (ab246510) at 1/1000 dilution
Lane 1 : NIH/3T3 (mouse embryonic fibroblast) treated at 37°C for 48 hours, whole cell lysate
Lane 2 : NIH/3T3 treated at 32°C for 48 hours, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
This antibody also recognized an undetermined band at around 50 kDa
Exposure time: 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (2)
ab246510 has been referenced in 2 publications.
- Liu W et al. Targeting extracellular CIRP with an X-aptamer shows therapeutic potential in acute pancreatitis. iScience 26:107043 (2023). PubMed: 37360693
- Xu Y et al. Human antigen R (HuR) and Cold inducible RNA-binding protein (CIRP) influence intestinal mucosal barrier function in ulcerative colitis by competitive regulation on Claudin1. Biofactors 47:427-443 (2021). PubMed: 33638934