Biotin Anti-CD45.1 antibody [A20] (ab25011)
Key features and details
- Biotin Mouse monoclonal [A20] to CD45.1
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Mouse
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Biotin Anti-CD45.1 antibody [A20]
See all CD45.1 primary antibodies -
Description
Biotin Mouse monoclonal [A20] to CD45.1 -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IP, ICC/IF, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse CD45.1. SJL mouse thymocytes and splenocytes
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General notes
ab25011 recognizes all mouse leukocytes expressing the CD45.1 allotype. It is useful as a marker for origin of donor and host cells
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
ab25011 recognizes all mouse leukocytes expressing the CD45.1 allotype. It is useful as a marker for origin of donor and host cells -
Clonality
Monoclonal -
Clone number
A20 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
- PerCP/Cy5.5® Anti-CD45.1 antibody [A20] (ab210237)
- violetFluor™ 450 Anti-CD45.1 antibody [A20] (ab241939)
- FITC Anti-CD45.1 antibody [A20] (ab24917)
- Cy5 ® Anti-CD45.1 antibody [A20] (ab24982)
- Anti-CD45.1 antibody [A20] (ab25078)
- APC Anti-CD45.1 antibody [A20] (ab25348)
- PE/Cy5® Anti-CD45.1 antibody [A20] (ab25349)
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18454 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
CD45 is an essential regulator of leukocyte activation and development, and is now known to be a protein tyrosine phosphatase, e.g. CD45 is able to dephosphorylate the Src family of protein kinases. This activity is now known to be required for signal transduction induced by T and B cell antigen receptor engagement, cytokine signaling and Fc receptor stimulation. The functional state of CD45 also has a pronounced effect on lymphocyte development. CD45 exists in multiple forms, such as the restricted variants and as allelic forms CD45.1/Ly5.1 and CD45.2/Ly5.2. The former arises as a result of alternative splicing of different exons. The specificity of antibodies to CD45.1 and CD45.2 has been exploited in studies in which resolution of donor and recipient cells is essential, such as in gene therapy or bone marrow transplantation. -
Cellular localization
Type I membrane protein. -
Database links
- Entrez Gene: 19264 Mouse
- SwissProt: P06800 Mouse
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Alternative names
- B220 antibody
- CD45 antibody
- CD45 antigen antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab25011 has not yet been referenced specifically in any publications.