Recombinant Anti-Sacsin antibody [EPR11864] - BSA and Azide free (ab250414)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11864] to Sacsin - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Sacsin antibody [EPR11864] - BSA and Azide free
See all Sacsin primary antibodies -
Description
Rabbit monoclonal [EPR11864] to Sacsin - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250414 is the carrier-free version of ab181190.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11864 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 521 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 521 kDa. |
Target
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Function
Co-chaperone which acts as a regulator of the Hsp70 chaperone machinery and may be involved in the processing of other ataxia-linked proteins. -
Tissue specificity
Highly expressed in the central nervous system. Also found in skeletal muscle and at low levels in pancreas. -
Involvement in disease
Spastic ataxia Charlevoix-Saguenay type -
Sequence similarities
Contains 1 HEPN domain.
Contains 1 J domain.
Contains 1 ubiquitin-like domain. -
Domain
The ubiquitin-like domain mediates interaction with the proteasome.
The J domain is functional and is shown to stimulate E.coli dnaK ATPase activity. -
Cellular localization
Cytoplasm. Predominantly cytoplasmic, a small portion is present in the nucleus and also shows a partial mitochondrial overlap with the mitochondrial marker Hsp60. - Information by UniProt
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Database links
- Entrez Gene: 26278 Human
- Omim: 604490 Human
- SwissProt: Q9NZJ4 Human
- Unigene: 159492 Human
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Alternative names
- DnaJ homolog subfamily C member 29 antibody
- DNAJC29 antibody
- SACS antibody
see all
Images
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Anti-Sacsin antibody [EPR11864] (ab181190) at 1/5000 dilution + Human glioma tissue lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform)This data was developed using ab181190, the same antibody clone in a different buffer formulation.
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Anti-Sacsin antibody [EPR11864] (ab181190) at 1/2000 dilution + Human fetal brain at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 521 kDa
Additional bands at: 437 kDa (possible isoform)This data was developed using ab181190, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250414 has not yet been referenced specifically in any publications.