Recombinant Anti-ACSM2A antibody [EPR13330-17] - BSA and Azide free (ab250534)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13330-17] to ACSM2A - BSA and Azide free
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-ACSM2A antibody [EPR13330-17] - BSA and Azide free
See all ACSM2A primary antibodies -
Description
Rabbit monoclonal [EPR13330-17] to ACSM2A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250534 is the carrier-free version of ab181865.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13330-17 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250534 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro). -
Sequence similarities
Belongs to the ATP-dependent AMP-binding enzyme family. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 123876 Human
- Entrez Gene: 233799 Mouse
- Entrez Gene: 246263 Rat
- Omim: 614358 Human
- SwissProt: Q08AH3 Human
- SwissProt: Q8K0L3 Mouse
- SwissProt: O70490 Rat
- Unigene: 298252 Human
see all -
Alternative names
- A-923A4.1 antibody
- ACS2A_HUMAN antibody
- ACSM2 antibody
see all
Images
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All lanes : Anti-ACSM2A antibody [EPR13330-17] (ab181865) at 1/10000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
Observed band size: 64 kDaThis data was developed using ab181865, the same antibody clone in a different buffer formulation.
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This data was developed using ab181865, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded, Human liver tissue labeling ACSM2A with ab181865 at a 1/50 dilution. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250534 has not yet been referenced specifically in any publications.