Recombinant Anti-PSMD9 antibody [EPR12947] - BSA and Azide free (ab250563)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12947] to PSMD9 - BSA and Azide free
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-PSMD9 antibody [EPR12947] - BSA and Azide free
See all PSMD9 primary antibodies -
Description
Rabbit monoclonal [EPR12947] to PSMD9 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, Flow Cyt (Intra)more details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250563 is the carrier-free version of ab181998.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12947 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-PSMD9 antibody [EPR12947] (ab181998)
- PE Anti-PSMD9 antibody [EPR12947] (ab305606)
- APC Anti-PSMD9 antibody [EPR12947] (ab305607)
- HRP Anti-PSMD9 antibody [EPR12947] (ab305608)
- Alexa Fluor® 488 Anti-PSMD9 antibody [EPR12947] (ab309752)
- Alexa Fluor® 647 Anti-PSMD9 antibody [EPR12947] (ab310119)
- Alexa Fluor® 594 Anti-PSMD9 antibody [EPR12947] (ab310528)
- Alexa Fluor® 555 Anti-PSMD9 antibody [EPR12947] (ab312058)
- Alexa Fluor® 568 Anti-PSMD9 antibody [EPR12947] (ab312535)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa).
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 25 kDa). |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
Target
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Function
Acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC). During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. -
Tissue specificity
Expressed in all tissues tested, highly expressed in liver and kidney. -
Sequence similarities
Belongs to the proteasome subunit p27 family.
Contains 1 PDZ (DHR) domain. - Information by UniProt
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Database links
- Entrez Gene: 5715 Human
- Entrez Gene: 67151 Mouse
- Entrez Gene: 161475 Rat
- Omim: 603146 Human
- SwissProt: O00233 Human
- SwissProt: Q9CR00 Mouse
- SwissProt: Q9WTV5 Rat
- Unigene: 131151 Human
see all -
Alternative names
- 26S proteasome non ATPase regulatory subunit 9 antibody
- 26S proteasome non-ATPase regulatory subunit 9 antibody
- 26S proteasome regulatory subunit p27 antibody
see all
Images
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All lanes : Anti-PSMD9 antibody [EPR12947] (ab181998) at 1/1000 dilution
Lane 1 : Human fetal liver tissue lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP at 1/1000 dilution
Predicted band size: 25 kDaThis data was developed using ab181998, the same antibody clone in a different buffer formulation.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling PSMD9 with ab181998 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling PSMD9 with ab181998 at 1/500 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary and counter-stained with DAPI (blue).
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling PSMD9 with ab181998 at 1/300 dilution (red) or a rabbit IgG (negative) (green), followed by secondary Goat anti-rabbit IgG (FITC) at 1/150 dilution.
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This data was developed using ab181998, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling PSMD9 with ab181998 at 1/500 dilution. A Goat anti rabbit IgG(Alexa Fluor® 555) was used as secondary antibody. Counterstained with DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250563 has not yet been referenced specifically in any publications.