Recombinant Anti-HLTF antibody [EPR14761] - BSA and Azide free (ab250652)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14761] to HLTF - BSA and Azide free
- Suitable for: IHC-P, WB, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-HLTF antibody [EPR14761] - BSA and Azide free
See all HLTF primary antibodies -
Description
Rabbit monoclonal [EPR14761] to HLTF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250652 is the carrier-free version of ab183042.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14761 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Conjugation kits
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250652 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa).
|
|
ICC/IF |
Use at an assay dependent concentration.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 114 kDa (predicted molecular weight: 114 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters (By similarity). These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA. -
Tissue specificity
Expressed in brain, heart, kidney, liver, lung, pancreas, placenta and skeletal muscle. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the SNF2/RAD54 helicase family. RAD16 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 RING-type zinc finger. -
Cellular localization
Cytoplasm. Nucleus. Nucleus, nucleolus. Nucleus, nucleoplasm. Nuclear localization is stimulated by progesterone. - Information by UniProt
-
Database links
- Entrez Gene: 6596 Human
- Entrez Gene: 295568 Rat
- Omim: 603257 Human
- SwissProt: Q14527 Human
- Unigene: 3068 Human
-
Alternative names
- DNA-binding protein/plasminogen activator inhibitor 1 regulator antibody
- Helicase like transcription factor antibody
- Helicase-like transcription factor antibody
see all
Images
-
All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1 µg
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : HLTF knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 114 kDa
Observed band size: 113 kDa why is the actual band size different from the predicted?This data was developed using ab183042, the same antibody clone in a different buffer formulation.
Lanes 1 - 3: Merged signal (red and green). Green - ab183042 observed at 113 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab183042 was shown to specifically react with HLTF in wild-type HAP1 cells as signal was lost in HLTF knockout cells. Wild-type and HLTF knockout samples were subjected to SDS-PAGE. ab183042 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
This data was developed using ab183042, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of Jurkat cells (-20°C Acetone-fixed) labeling HLTF with ab183042 at 1/100 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
-
All lanes : Anti-HLTF antibody [EPR14761] (ab183042) at 1/50000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 lysate
Lane 3 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 114 kDaThis data was developed using ab183042, the same antibody clone in a different buffer formulation.
-
This data was developed using ab183042, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
This data was developed using ab183042, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human infiltrating duct carcinoma of breast tissue labeling HLTF with ab183042 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250652 has not yet been referenced specifically in any publications.