Recombinant Anti-Plakophilin 1 antibody [EPR14890] - BSA and Azide free (ab250665)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14890] to Plakophilin 1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-Plakophilin 1 antibody [EPR14890] - BSA and Azide free
See all Plakophilin 1 primary antibodies -
Description
Rabbit monoclonal [EPR14890] to Plakophilin 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250665 is the carrier-free version of ab183512.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14890 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 81 kDa).
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 75 kDa (predicted molecular weight: 81 kDa). |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Seems to play a role in junctional plaques. Contributes to epidermal morphogenesis. -
Tissue specificity
Isoform 2 is widely expressed. Isoform 1 is expressed in stratified squamous, complex, glandular duct and bladder epithelia. -
Involvement in disease
Ectodermal dysplasia-skin fragility syndrome -
Sequence similarities
Belongs to the beta-catenin family.
Contains 9 ARM repeats. -
Cellular localization
Nucleus and Nucleus. Cell junction, desmosome. - Information by UniProt
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Database links
- Entrez Gene: 5317 Human
- Entrez Gene: 18772 Mouse
- Omim: 601975 Human
- SwissProt: Q13835 Human
- SwissProt: P97350 Mouse
- Unigene: 497350 Human
- Unigene: 4494 Mouse
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Alternative names
- B6P antibody
- Band 6 protein antibody
- PKP1 antibody
see all
Images
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All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/20000 dilution
Lane 1 : Mouse skin tissue lysate
Lane 2 : A431 cell lysate
Lane 3 : HACAT cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab183512, the same antibody clone in a different buffer formulation.
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All lanes : Anti-Plakophilin 1 antibody [EPR14890] (ab183512) at 1/5000 dilution
Lane 1 : Human skin tissue lysate
Lane 2 : Human tonsil tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 81 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?This data was developed using ab183512, the same antibody clone in a different buffer formulation.
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This data was developed using ab183512, the same antibody clone in a different buffer formulation.Immunofluorescent staining of 4% paraformaldehyde fixed HACAT cells labeling Plakophilin 1 using ab183512 at a 1/100 dilution followed by Goat anti Rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution. Counterstained with Dapi (bue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250665 has not yet been referenced specifically in any publications.