Recombinant Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free (ab250679)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Angiopoietin 1 antibody [EPR2888(N)] - BSA and Azide free
See all Angiopoietin 1 primary antibodies -
Description
Rabbit monoclonal [EPR2888(N)] to Angiopoietin 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: U87-MG, HeLa, C6, RAW 264.7, K562, and ECV-304 cell lysates.
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General notes
ab250679 is the carrier-free version of ab183701.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2888(N) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250679 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development. -
Sequence similarities
Contains 1 fibrinogen C-terminal domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 284 Human
- Entrez Gene: 11600 Mouse
- Entrez Gene: 89807 Rat
- Omim: 601667 Human
- SwissProt: Q15389 Human
- SwissProt: O08538 Mouse
- SwissProt: O35460 Rat
- Unigene: 369675 Human
see all -
Alternative names
- AGP 1 antibody
- AGP1 antibody
- AGPT antibody
see all
Images
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All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/10000 dilution (Purified)
Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell) whole cell lysates
Lane 2 : C6 (Rat glial tumor glial cell) whole cell lysates
Lane 3 : RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates
Lane 4 : Human brain lysates
Lane 5 : Mouse brain lysates
Lane 6 : Rat brain lysates
Lane 7 : Human heart lysates
Lane 8 : Mouse heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?This data was developed using ab183701, the same antibody clone in a different buffer formulation.
This antibody is unsuitable to detect tissue lysate samples.
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All lanes : Anti-Angiopoietin 1 antibody [EPR2888(N)] (ab183701) at 1/50000 dilution
Lane 1 : U87-MG cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : ECV-304 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 57 kDaThis data was developed using ab183701, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250679 has not yet been referenced specifically in any publications.