Recombinant Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] - BSA and Azide free (ab250755)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA) - BSA and Azide free
- Suitable for: Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] - BSA and Azide free
See all Protective protein/Cathepsin A (PPCA) primary antibodies -
Description
Rabbit monoclonal [EPR10435] to Protective protein/Cathepsin A (PPCA) - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250755 is the carrier-free version of ab184553.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10435 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553)
- Alexa Fluor® 488 Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab309838)
- Alexa Fluor® 647 Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab310206)
- Alexa Fluor® 555 Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab312179)
- Alexa Fluor® 568 Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab312666)
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250755 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
Target
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Function
Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. -
Involvement in disease
Defects in CTSA are the cause of galactosialidosis (GSL) [MIM:256540]. A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. -
Sequence similarities
Belongs to the peptidase S10 family. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 5476 Human
- Entrez Gene: 19025 Mouse
- Entrez Gene: 296370 Rat
- Omim: 613111 Human
- SwissProt: P10619 Human
- SwissProt: P16675 Mouse
- Unigene: 609336 Human
- Unigene: 359633 Mouse
see all -
Alternative names
- beta galactosidase 2 antibody
- BETA GALACTOSIDASE PROTECTIVE PROTEIN antibody
- beta-galactosidase 2 antibody
see all
Images
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All lanes : Anti-Protective protein/Cathepsin A (PPCA) antibody [EPR10435] (ab184553) at 1/20000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 54 kDa
Observed band size: 54 kDaThis data was developed using ab184553, the same antibody clone in a different buffer formulation.
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This data was developed using ab184553, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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This data was developed using ab184553, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling Protective protein/Cathepsin A (PPCA) with ab184553 at 1/140 dilution (red) compared with a Rabbit monoclonal IgG isotype control (green), followed by Goat anti-rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250755 has not yet been referenced specifically in any publications.