Recombinant Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free (ab250820)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13380] to SDHAF1 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SDHAF1 antibody [EPR13380] - BSA and Azide free
See all SDHAF1 primary antibodies -
Description
Rabbit monoclonal [EPR13380] to SDHAF1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250820 is the carrier-free version of ab185222.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13380 -
Isotype
IgG
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa).
This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 21 kDa (predicted molecular weight: 13 kDa). This product detects the target protein in mitochondrial lysates (17 kDa). In our hands we are unable to detect the target protein in whole cell lysates.
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Target
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Function
Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II. -
Tissue specificity
Ubiquitously expressed. -
Pathway
Cofactor biosynthesis; iron-sulfur cluster biosynthesis. -
Involvement in disease
Mitochondrial complex II deficiency -
Sequence similarities
Belongs to the complex I LYR family. SDHAF1 subfamily. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 644096 Human
- Entrez Gene: 68332 Mouse
- Entrez Gene: 100912030 Rat
- Entrez Gene: 499125 Rat
- Omim: 612848 Human
- SwissProt: A6NFY7 Human
- SwissProt: Q3U276 Mouse
- SwissProt: B0K036 Rat
see all -
Alternative names
- LYR motif containing 8 antibody
- LYR motif-containing protein 8 antibody
- LYRM8 antibody
see all
Images
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This data was developed using ab185222, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 293 cells (4% paraformaldehyde-fixed) labeling SDHAF1 with ab185222 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution and Counterstained with DAPI (blue).
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All lanes : Anti-SDHAF1 antibody [EPR13380] (ab185222) at 1/1000 dilution
Lane 1 : MCF7 whole cell lysate
Lane 2 : MCF7 mitochondria fraction lysate
Lane 3 : MCF7 mitochondria control lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesThis data was developed using ab185222, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250820 has not yet been referenced specifically in any publications.