Recombinant Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free (ab250838)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8756] to DNAJA4 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), IP, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DNAJA4 antibody [EPR8756] - BSA and Azide free
See all DNAJA4 primary antibodies -
Description
Rabbit monoclonal [EPR8756] to DNAJA4 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), IP, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab250838 is the carrier-free version of ab185553.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8756 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Sequence similarities
Contains 1 CR-type zinc finger.
Contains 1 J domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 55466 Human
- Entrez Gene: 58233 Mouse
- Entrez Gene: 300721 Rat
- SwissProt: Q8WW22 Human
- SwissProt: Q9JMC3 Mouse
- Unigene: 513053 Human
- Unigene: 489676 Mouse
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Alternative names
- DnaJ (Hsp40) homolog subfamily A member 4 antibody
- DnaJ (Hsp40) homolog, subfamily A, member4 antibody
- DNAJ A4 antibody
see all
Images
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All lanes : Anti-DNAJA4 antibody [EPR8756] (ab185553) at 1/10000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Hela cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 45 kDaThis data was developed using ab185553, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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This data was developed using ab185553, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of DNAJA4 in 2% paraformaldehyde HeLa cells using ab185553 at 1/300 dilution (pink). Secondary antibody: Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control: Rabbit monoclonal IgG (green).
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This data was developed using ab185553, the same antibody clone in a different buffer formulation.Western blot analysis of Immunoprecipitate from Human fetal brain lysate (lane 1) using ab185553 at 1/50 dilution. Lane 2: PBS used instead of Human fetal brain lysates. Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, at 1/1500 dilution. Blocking buffer: 5% NFDM/TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab250838 has not yet been referenced specifically in any publications.