Recombinant Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free (ab250844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17744] to Cytokeratin 1 - BSA and Azide free
- Suitable for: IHC-Fr, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-Cytokeratin 1 antibody [EPR17744] - BSA and Azide free
See all Cytokeratin 1 primary antibodies -
Description
Rabbit monoclonal [EPR17744] to Cytokeratin 1 - BSA and Azide free -
Host species
Rabbit -
Specificity
Recombinant mouse cytokeratin 5 could be detected at very high antibody concentrations in WB.
-
Tested applications
Suitable for: IHC-Fr, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
ab250844 is the carrier-free version of ab185628.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17744 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
- Anti-Cytokeratin 1 antibody [EPR17744] (ab185628)
- Alexa Fluor® 647 Anti-Cytokeratin 1 antibody [EPR17744] (ab311147)
- Alexa Fluor® 555 Anti-Cytokeratin 1 antibody [EPR17744] (ab311324)
- Alexa Fluor® 594 Anti-Cytokeratin 1 antibody [EPR17744] (ab311789)
- Alexa Fluor® 568 Anti-Cytokeratin 1 antibody [EPR17744] (ab313070)
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab250844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Use at an assay dependent concentration.
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
WB |
Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa).
|
Notes |
---|
IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). |
Target
-
Function
May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein kinase C (RACK1/GNB2L1). -
Tissue specificity
The source of this protein is neonatal foreskin. The 67-kDa type II keratins are expressed in terminally differentiating epidermis. -
Involvement in disease
Defects in KRT1 are a cause of bullous congenital ichthyosiform erythroderma (BCIE) [MIM:113800]; also known as epidermolytic hyperkeratosis (EHK) or bullous erythroderma ichthyosiformis congenita of Brocq. BCIE is an autosomal dominant skin disorder characterized by widespread blistering and an ichthyotic erythroderma at birth that persist into adulthood. Histologically there is a diffuse epidermolytic degeneration in the lower spinous layer of the epidermis. Within a few weeks from birth, erythroderma and blister formation diminish and hyperkeratoses develop.
Defects in KRT1 are the cause of ichthyosis hystrix Curth-Macklin type (IHCM) [MIM:146590]. IHCM is a genodermatosis with severe verrucous hyperkeratosis. Affected individuals manifest congenital verrucous black scale on the scalp, neck, and limbs with truncal erythema, palmoplantar keratoderma and keratoses on the lips, ears, nipples and buttocks.
Defects in KRT1 are a cause of palmoplantar keratoderma non-epidermolytic (NEPPK) [MIM:600962]. NEPKK is a dermatological disorder characterized by focal palmoplantar keratoderma with oral, genital, and follicular lesions.
Defects in KRT1 are a cause of ichthyosis annular epidermolytic (AEI) [MIM:607602]; also known as cyclic ichthyosis with epidermolytic hyperkeratosis. AEI is a skin disorder resembling bullous congenital ichthyosiform erythroderma. Affected individuals present with bullous ichthyosis in early childhood and hyperkeratotic lichenified plaques in the flexural areas and extensor surfaces at later ages. The feature that distinguishes AEI from BCIE is dramatic episodes of flares of annular polycyclic plaques with scale, which coalesce to involve most of the body surface and can persist for several weeks or even months.
Defects in KRT1 are the cause of palmoplantar keratoderma striate type 3 (SPPK3) [MIM:607654]; also known as keratosis palmoplantaris striata III. SPPK3 is a dermatological disorder affecting palm and sole skin where stratum corneum and epidermal layers are thickened. There is no involvement of non-palmoplantar skin, and both hair and nails are normal. -
Sequence similarities
Belongs to the intermediate filament family. -
Post-translational
modificationsUndergoes deimination of some arginine residues (citrullination). -
Cellular localization
Cell membrane. Located on plasma membrane of neuroblastoma NMB7 cells. - Information by UniProt
-
Database links
- Entrez Gene: 3848 Human
- Entrez Gene: 16678 Mouse
- Entrez Gene: 300250 Rat
- Omim: 139350 Human
- SwissProt: P04264 Human
- SwissProt: P04104 Mouse
- SwissProt: Q6IMF3 Rat
- Unigene: 80828 Human
see all -
Alternative names
- 67 kDa cytokeratin antibody
- CK-1 antibody
- CK1 antibody
see all
Images
-
This data was developed using ab185628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse skin is observed (PMID: 12010363). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab185628, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative Control: No staining on mouse stomach. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. -
This data was developed using ab185628, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat skin is observed (PMID: 12010363). Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab185628, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling Cytokeratin 1 with ab185628 at 1/600 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Negative Control: No staining on rat kidney. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
-
This data was developed using ab185628, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse skin tissue section labeling Cytokeratin 1 with ab185628 at 1/200 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). The result showed cytoplasmic staining on mouse skin. The nuclear counter stain is DAPI (blue). Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
-
All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution
Lane 1 : Mouse skin lysate
Lane 2 : Mouse brain lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Mouse heart lysate
Lane 5 : Rat skin lysate
Lane 6 : Rat brain lysate
Lane 7 : Rat spleen lysate
Lane 8 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 9 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDaThis data was developed using ab185628, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-4: 30 seconds; Lanes 5-9: 3 minutes.
Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).
-
All lanes : Anti-Cytokeratin 1 antibody [EPR17744] (ab185628) at 1/20000 dilution
Lane 1 : Human skin lysate
Lane 2 : Human fetal brain lysate
Lane 3 : Human fetal heart lysate
Lane 4 : Human fetal kidney lysate
Lane 5 : Human spleen lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3 minutesThis data was developed using ab185628, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Cytokeratin 1 is expressed in terminally differentiating epidermis (PMID: 2580302).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
Datasheet download
Certificate of Compliance
References (0)
ab250844 has not yet been referenced specifically in any publications.