Anti-CD16+CD32 antibody [93] - BSA and Azide free (ab25235)
Key features and details
- Rat monoclonal [93] to CD16+CD32 - BSA and Azide free
- Suitable for: Flow Cyt, Blocking, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-CD16+CD32 antibody [93] - BSA and Azide free
See all CD16+CD32 primary antibodies -
Description
Rat monoclonal [93] to CD16+CD32 - BSA and Azide free -
Host species
Rat -
Tested applications
Suitable for: Flow Cyt, Blocking, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus. Sorted Mouse pre-B cells
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Epitope
ab25235 reacts with a conformational epitope formed by CD16 Fc gamma II and CD32 Fc gamma III receptors. -
Positive control
- ICC: HeLa cells Flow Cyt: C57BL/6 splenocytes.
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General notes
This product was changed from ascites to tissue culture supernatant on 02/08/2019. Lot numbers higher than GR3269503 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
93 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25235 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use a concentration of 5 µg/ml.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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Blocking |
Use at an assay dependent concentration.
ab25235 can be used for blocking of Fc gamma receptors. |
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
Notes |
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Flow Cyt
Use a concentration of 5 µg/ml. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Blocking
Use at an assay dependent concentration. ab25235 can be used for blocking of Fc gamma receptors. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Relevance
Function: Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. Tissue specificity: Found on monocytes, neutrophils and eosinophil platelets. Similarity: Contains 2 Ig-like C2-type (immunoglobulin-like) domains. PTM: Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. -
Cellular localization
CD32: Type I membrane protein. CD16: Attached to the membrane by a GPI anchor. Exists also as a soluble receptor, produced by a proteolytic cleavage. -
Database links
- Entrez Gene: 2212 Human
- Entrez Gene: 2213 Human
- Entrez Gene: 2214 Human
- Entrez Gene: 2215 Human
- Entrez Gene: 14130 Mouse
- Entrez Gene: 14131 Mouse
- Omim: 146740 Human
- SwissProt: O75015 Human
see all -
Alternative names
- CD16a antibody
- CD16b antibody
- CD32 antibody
see all
Images
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C57BL/6 mouse splenocytes stained with ab25235 (right) or Rat IgG2aκ (ab18450) isotype (left). C57BL/6 mouse splenocytes were incubated for 30 min on ice in PBS / 10 % mouse serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab25235) or Rat IgG2aκ (ab18450) (1x106 in 100µl at 5 µg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor ® 488, pre-adsorbed) (ab150165) was used at 1/2000 dilution for 30 min at 4°C. The cells were simultaneously stained with CD19 antibody.
Acquisition of >30,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. Events were gated on viable lymphocytes.
This image was generated using the ascites version of the product.
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ab25235 staining CD16 + CD32 in Raw264.7 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab25235 at 10µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab25235 has been referenced in 14 publications.
- Misilimu D et al. Intranasal Salvinorin A Improves Long-term Neurological Function via Immunomodulation in a Mouse Ischemic Stroke Model. J Neuroimmune Pharmacol 17:350-366 (2022). PubMed: 34596819
- Honig MG et al. Raloxifene Modulates Microglia and Rescues Visual Deficits and Pathology After Impact Traumatic Brain Injury. Front Neurosci 15:701317 (2021). PubMed: 34776838
- Wu H et al. Mer regulates microglial/macrophage M1/M2 polarization and alleviates neuroinflammation following traumatic brain injury. J Neuroinflammation 18:2 (2021). PubMed: 33402181
- Zheng J et al. Ceria nanoparticles ameliorate white matter injury after intracerebral hemorrhage: microglia-astrocyte involvement in remyelination. J Neuroinflammation 18:43 (2021). PubMed: 33588866
- Wang M et al. The Ectopic Expression of SurvivinT34A and FilC Can Enhance the Oncolytic Effects of Vaccinia Virus in Murine Gastric Cancer. Onco Targets Ther 13:1011-1025 (2020). PubMed: 32099404