Recombinant Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] -
Description
Rabbit monoclonal [EPR22455-57] to SerpinB3/SCCA + SerpinB4/SCCA-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A431 whole cell lysate. Human fetal lung tissue lysate. IHC-P: Human pancreatic carcinoma and ovarian cancer tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22455-57 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254255 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
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IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Cellular localization
SerpinB3/SCCA: Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level. SerpinB4/SCCA-2: Cytoplasm. Seems to also be secreted in plasma by cancerous cells but at a low level. -
Database links
- Entrez Gene: 6317 Human
- Entrez Gene: 6318 Human
- Omim: 600517 Human
- Omim: 600518 Human
- SwissProt: P29508 Human
- SwissProt: P48594 Human
- Unigene: 123035 Human
- Unigene: 227948 Human
Images
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Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma and the paired adjacent pancreas labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID: 24759783) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254255 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lane 2 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 3 : Human fetal lung tissue lysate
Lane 4 : Human liver tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDa
Exposure time: 3 minutesThe expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:24517394).
Negative control: Human liver (PMID:28611447).
HepG2 as a low serpinB expressing cell line.
This blot was developed using a higher sensitivity ECL substrate.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human ovarian cancer labeling SerpinB3/SCCA with ab254255 at 1/500 dilution, followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on ovarian cancer (PMID: 23185467) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with ab254255 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-SerpinB3/SCCA + SerpinB4/SCCA-2 antibody [EPR22455-57] (ab254255) at 1/1000 dilution
Lane 1 : Recombinant Human Serpin B3/SSCA1 Protein 20 ng
Lane 2 : Recombinant Human Serpin B4/SCCA-2 Protein 20 ng
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 45 kDa
Observed band size: 45 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3.25 secs.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab254255 has not yet been referenced specifically in any publications.