Recombinant Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22642-17] to Asialoglycoprotein Receptor 1/HL-1
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17]
See all Asialoglycoprotein Receptor 1/HL-1 primary antibodies -
Description
Rabbit monoclonal [EPR22642-17] to Asialoglycoprotein Receptor 1/HL-1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human adult liver tissue lysate; HepG2 cell lysate. IHC-P: Human liver and liver cancer tissue. ICC/IF: HepG2 cells. Flow: HepG2 cells. IP: HepG2 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22642-17 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab254262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 33 kDa).
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ICC/IF |
1/100.
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IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Flow Cyt |
1/500.
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IP |
1/30.
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Notes |
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WB
1/1000. Detects a band of approximately 40-50 kDa (predicted molecular weight: 33 kDa). |
ICC/IF
1/100. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt
1/500. |
IP
1/30. |
Target
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Function
Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface. -
Tissue specificity
Expressed exclusively in hepatic parenchymal cells. -
Sequence similarities
Contains 1 C-type lectin domain. -
Post-translational
modificationsPhosphorylated on a cytoplasmic Ser residue. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 432 Human
- Omim: 108360 Human
- SwissProt: P07306 Human
- Unigene: 12056 Human
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Alternative names
- ASGP-R 1 antibody
- ASGPR 1 antibody
- ASGPR antibody
see all
Images
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All lanes : Anti-Asialoglycoprotein Receptor 1/HL-1 antibody [EPR22642-17] (ab254262) at 1/1000 dilution
Lane 1 : Human adult liver tissue lysate
Lane 2 : Human brain tissue lysate
Lane 3 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 33 kDa
Observed band size: 40-50 kDa why is the actual band size different from the predicted?Negative control: human brain.
The diffuse bands between 40-50 kDa are likely due to glycosylation (PMID:30965276).
The expression profile is consistent with what has been described in the literature (PMID:27241665, 29858079).
Blocking/diluting buffer and concentration: 5% NFDM/TBST
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Asialoglycoprotein Receptor 1/HL-1 antibody was immunoprecipitated from 0.35 mg HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg with ab254262 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab254262 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate 10μg
Lane 2: ab254262 IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab254262 in HepG2 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7 seconds.
The band at around 100kDa is described as dimers (PMID: 7663158).
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human hepatocellular carcinoma epithelial cell line) cells labelling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/100 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous and weak cytoplasmic staining in HepG2 cells. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counter stain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver (PMID:23979840, 26422581, 27241665) is observed.
The section was incubated with ab254262 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human liver cancer (PMID:23979840,26422581,27241665) is observed.
The section was incubated with ab254262 for 15 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Negative control: No staining in human colon (PMID:23979840).
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab254262 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Negative control: No staining in human cerebrum (PMID:23979840)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/1000 dilution (0.5 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
The section was incubated with ab254262 for 15 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Flow cytometric analysis of HepG2 (Human hepatocellular carcinoma epithelial cell line) cells labelling Asialoglycoprotein Receptor 1/HL-1 antibody with ab254262 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730, Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab254262 has been referenced in 3 publications.
- You J et al. Hepatic exosomes with declined MiR-27b-3p trigger RIG-I/TBK1 signal pathway in macrophages. Liver Int 42:1676-1691 (2022). PubMed: 35460174
- Li N et al. Astragaloside IV alleviates silica-induced pulmonary fibrosis via inactivation of the TGF-ß1/Smad2/3 signaling pathway. Int J Mol Med 47:N/A (2021). PubMed: 33448318
- Wu SY et al. Low molecular weight fucoidan inhibits hepatocarcinogenesis and nonalcoholic fatty liver disease in zebrafish via ASGR/STAT3/HNF4A signaling. Clin Transl Med 10:e252 (2020). PubMed: 33377648