Recombinant Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-ErbB3 / HER3 antibody [EPR22669-25]
See all ErbB3 / HER3 primary antibodies -
Description
Rabbit monoclonal [EPR22669-25] to ErbB3 / HER3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt (Intra)more details
Unsuitable for: ICC/IF,IHC-Fr or IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MCF7, 4T1, SK-BR-3 and T-47D whole cell lysate. Mouse stomach and skin tissue lysate. IHC-P: Human bladder cancer and breast cancer tissue. Mouse skin tissue. Flow Cyt (intra): MCF7 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22669-25 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry reagents
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab255607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000. Predicted molecular weight: 148 kDa.
Fresh lysates are needed for western blot. |
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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|
Flow Cyt (Intra) |
1/50.
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Notes |
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WB
1/1000. Predicted molecular weight: 148 kDa. Fresh lysates are needed for western blot. |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/50. |
Target
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Function
Binds and is activated by neuregulins and NTAK. -
Tissue specificity
Epithelial tissues and brain. -
Involvement in disease
Defects in ERBB3 are the cause of lethal congenital contracture syndrome type 2 (LCCS2) [MIM:607598]; also called Israeli Bedouin multiple contracture syndrome type A. LCCS2 is an autosomal recessive neurogenic form of a neonatally lethal arthrogryposis that is associated with atrophy of the anterior horn of the spinal cord. The LCCS2 syndrome is characterized by multiple joint contractures, anterior horn atrophy in the spinal cord, and a unique feature of a markedly distended urinary bladder. The phenotype suggests a spinal cord neuropathic etiology. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
Contains 1 protein kinase domain. -
Developmental stage
Overexpressed in a subset of human mammary tumors. -
Domain
The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal-transducing proteins. -
Post-translational
modificationsLigand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 2065 Human
- Entrez Gene: 13867 Mouse
- Omim: 190151 Human
- SwissProt: P21860 Human
- SwissProt: Q61526 Mouse
- Unigene: 118681 Human
- Unigene: 373043 Mouse
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Alternative names
- c erbB 3 antibody
- c erbB3 antibody
- Erb b2 receptor tyrosine kinase 3 antibody
see all
Images
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All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : T-47D (Human ductal breast epithelial tumor epithelial cell) whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 4 : MDA-MB-231 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 148 kDa
Exposure time: 26 secondsFresh lysates are needed for western blot.
ErbB 3 low expression cell lines: HeLa, MDA-MB-231 (PMID: 22436610, 22301547).
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining in human urothelial carcinoma (PMID: 23333248) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
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Intracellular flow cytometric analysis of 4% paraformaldehydefixed 90% methanolpermeabilized MDA-MB-231 (Human breast adenocarcinoma epithelial cell, Left) / MCF7 (Human breast adenocarcinoma epithelial cell, Right) cells labeling ErbB3 / HER3 with ab255607 at 1/50 dilution (1µg)/ Red compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Weak expression control: MDA-MB-231 (PMID: 25018110, 16519802).
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All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : 4T1 (Mouse mammary gland carcinoma epithelial cell) whole cell lysate
Lane 2 : SK-BR-3 (Human breast adenocarcinoma epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 148 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in mouse skin (PMID:26194695) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
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All lanes : Anti-ErbB3 / HER3 antibody [EPR22669-25] (ab255607) at 1/1000 dilution
Lane 1 : Mouse stomach lysate
Lane 2 : Mouse skin lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 148 kDa
Exposure time: 70 secondsThe expression profile/molecular weight observed is consistent with what has been described in the literature. PMID: 26194695 and 19690388.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling ErbB3 / HER3 with ab255607 at 1/250 dilution (2.1 μg/ml) followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Membranous staining in human breast carcinoma (PMID: 24346286) is observed. Counterstained with hematoxylin. Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab255607 has been referenced in 1 publication.
- Song S et al. EGFR/MET promotes hepatocellular carcinoma metastasis by stabilizing tumor cells and resisting to RTKs inhibitors in circulating tumor microemboli. Cell Death Dis 13:351 (2022). PubMed: 35428350