Recombinant Anti-PVRIG/CD112R antibody [EPR23797-104] (ab256808)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23797-104] to PVRIG/CD112R
- Suitable for: Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-PVRIG/CD112R antibody [EPR23797-104]
See all PVRIG/CD112R primary antibodies -
Description
Rabbit monoclonal [EPR23797-104] to PVRIG/CD112R -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- Flow cyt: HEK-293T and human PBMC cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23797-104 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab256808 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
1/500.
|
Notes |
---|
Flow Cyt
1/500. |
Target
-
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 79037 Human
- SwissProt: Q6DKI7 Human
- Unigene: 729356 Human
-
Alternative names
- Poliovirus receptor-related immunoglobulin domain-containing protein antibody
- PVRIG antibody
- PVRIG_HUMAN antibody
- Transmembrane protein PVRIG antibody
Images
-
Flow cytometric analysis of 2% paraformaldehyde fixed, 0.1% Tween-20 permeabilized HEK-293T (Human embryonic kidney epithelial cell) transfected with myc-tagged PVRIG/CD112R construct cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were surface stained with rabbit IgG and ab256808, then fixed with 2% PFA followed by intracellularly staining with anti-myc tag conjugated to AlexaFluor®647.
-
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling PVRIG/CD112R with ab256808 at 1/500 dilution (0.1ug)(Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with rabbit IgG or ab256808. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab256808 has not yet been referenced specifically in any publications.