Recombinant Anti-THBS4 antibody [EPR22922-232] (ab263898)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22922-232] to THBS4
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-THBS4 antibody [EPR22922-232]
See all THBS4 primary antibodies -
Description
Rabbit monoclonal [EPR22922-232] to THBS4 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human skeletal muscle, human serum, mouse skeletal muscle, human articular cartilage, mouse kidney, mouse articular cartilage, rat heart and rat articular cartilage lysates. ICC/IF: C2C12 and L6 cells. Flow Cyt (intra): C2C12 and L6 cells. IP: Human and mouse skeletal muscle whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22922-232 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab263898 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/700.
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WB |
1/1000. Predicted molecular weight: 106 kDa.
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ICC/IF |
1/100.
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IP |
1/30.
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Notes |
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Flow Cyt (Intra)
1/700. |
WB
1/1000. Predicted molecular weight: 106 kDa. |
ICC/IF
1/100. |
IP
1/30. |
Target
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Function
Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions. Can bind to fibrinogen, fibronectin, laminin and type V collagen. -
Sequence similarities
Belongs to the thrombospondin family.
Contains 4 EGF-like domains.
Contains 1 TSP C-terminal (TSPC) domain.
Contains 1 TSP N-terminal (TSPN) domain.
Contains 8 TSP type-3 repeats. - Information by UniProt
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Database links
- Entrez Gene: 7060 Human
- Entrez Gene: 21828 Mouse
- Entrez Gene: 29220 Rat
- Omim: 600715 Human
- SwissProt: P35443 Human
- SwissProt: Q9Z1T2 Mouse
- SwissProt: P49744 Rat
- Unigene: 211426 Human
see all -
Alternative names
- Thbs4 antibody
- thrombospondin 4 antibody
- thrombospondin 4b antibody
see all
Images
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All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution
Lane 1 : Human articular cartilage lysate
Lane 2 : Mouse kidney lysate
Lane 3 : Mouse articular cartilage lysate
Lane 4 : Rat heart lysate
Lane 5 : Rat articular cartilage lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lane 1 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lanes 2-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 106 kDaThe expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).
Exposure times: Lanes 1-4: 15 seconds; Lane 5: 6 seconds.
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All lanes : Anti-THBS4 antibody [EPR22922-232] (ab263898) at 1/1000 dilution
Lane 1 : Human skeletal muscle lysate
Lane 2 : Human serum lysate
Lane 3 : Mouse skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Lane 3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 106 kDa
Observed band size: 100,120,140,75 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile & molecular weight observed is consistent with what has been described in the literature (PMID: 17182969, 27581066, 30669608, 7852353, 12663449).
Exposure times: Lane 1: 48 secs; Lane 2: 6 secs; Lane 3: 1 sec.
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THBS4 was immunoprecipitated from 0.35 mg mouse skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse skeletal muscle lysate 10µg
Lane 2: ab263898 IP in mouse skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in mouse skeletal muscle lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
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THBS4 was immunoprecipitated from 0.35 mg human skeletal muscle lysate 10µg with ab263898 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab263898 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Human skeletal muscle lysate 10µg
Lane 2: ab263898 IP in human skeletal muscle lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab263868 in human skeletal muscle lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
The expression profile and molecular weight observed is consistent with what has been described in the literature (PMID:30669608).
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in L6 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C2C12 (Mouse myoblast) cells labeling THBS4 with ab263898 at 1/100 (6.9 µg/ml) dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 (2 µg/ml) dilution (Green). Confocal image showing cytoplasmic staining in C2C12 cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized L6 (Rat skeletal muscle myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized C2C12 (Mouse myoblasts myoblast) cells labeling THBS4 with ab263898 at 1/700 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (3)
ab263898 has been referenced in 3 publications.
- Best KT et al. Scleraxis-lineage cell depletion improves tendon healing and disrupts adult tendon homeostasis. Elife 10:N/A (2021). PubMed: 33480357
- Du S et al. The Attenuating Effect of Low-Intensity Pulsed Ultrasound on Hypoxia-Induced Rat Chondrocyte Damage in TMJ Osteoarthritis Based on TMT Labeling Quantitative Proteomic Analysis. Front Pharmacol 12:752734 (2021). PubMed: 34970140
- He SK et al. Segmentally Demineralized Cortical Bone With Stem Cell-Derived Matrix Promotes Proliferation, Migration and Differentiation of Stem Cells in vitro. Front Cell Dev Biol 9:776884 (2021). PubMed: 35155445